製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CoREST
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Zebrafish
  • 免疫原

    A region within synthetic peptide: MVEKGPEVSG KRRGRNNAAA SASAAAASAA ASAACASPAA TAASGAAASS, corresponding to N terminal amino acids 1-50 of Human CoREST

  • ポジティブ・コントロール
    • Jurkat cell lysate (Western blot). Human Lung (IHC-P).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab56165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the CoREST family.
    Contains 1 ELM2 domain.
    Contains 2 SANT domains.
  • ドメイン
    The SANT domains may bridge the nucleosomal substrates and the demethylase KDM1A.
  • 翻訳後修飾
    Phosphorylated by HSV-1 protein kinases in case of infection.
  • 細胞内局在
    Nucleus. Upon infection by HSV-1, it is partially translocated into the cytoplasm in an HSV-1-dependent manner.
  • Information by UniProt
  • 参照データベース
  • 別名
    • COREST antibody
    • KIAA0071 antibody
    • Nr2b2 antibody
    • Nuclear receptor coregulator 1 antibody
    • Nuclear receptor subfamily 2 group B member 2 antibody
    • Protein CoREST antibody
    • RCOR antibody
    • Rcor1 antibody
    • RCOR1_HUMAN antibody
    • REST corepressor 1 antibody
    • Retinoic acid receptor RXR-beta antibody
    • Retinoid X receptor beta antibody
    • Rxrb antibody
    see all

画像

  • Anti-CoREST antibody (ab56165) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling CoREST with ab56165 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

参考文献

This product has been referenced in:
  • Packer AN  et al. The bifunctional microRNA miR-9/miR-9* regulates REST and CoREST and is downregulated in Huntington's disease. J Neurosci 28:14341-6 (2008). Read more (PubMed: 19118166) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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