製品の概要

  • 製品名Anti-COPT2 antibody
  • 製品の詳細
    Rabbit polyclonal to COPT2
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to C-terminal residues of human COPT2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Involved in low-affinity copper uptake.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI604396 antibody
    • Copper transporter 2 antibody
    • COPT2 antibody
    • COPT2_HUMAN antibody
    • CTR2 antibody
    • hCTR2 antibody
    • MGC117640 antibody
    • Probable low affinity copper uptake protein 2 antibody
    • RP23 64F17.2 antibody
    • SLC31A2 antibody
    • Solute carrier family 31 member 2 antibody
    • Solute carrier family 31, member 2 antibody
    see all

Anti-COPT2 antibody 画像

  • ICC/IF image of ab58777 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58777, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-COPT2 antibody (ab58777) 使用論文

ab58777 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"