Anti-COPS6 抗体 (ab77299)
Key features and details
- Rabbit polyclonal to COPS6
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-COPS6 antibody
COPS6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to COPS6 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Pig, Zebrafish, Orangutan -
免疫原
Synthetic peptide corresponding to Human COPS6 aa 150-250.
(Peptide available asab92686) -
ポジティブ・コントロール
- This antibody gave a positive signal in NIH3T3 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77299の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | ||
WB |
特記事項 |
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WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Has some glucocorticoid receptor-responsive activity. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the peptidase M67A family. CSN6 subfamily.
Contains 1 MPN (JAB/Mov34) domain. -
細胞内局在
Nucleus. Cytoplasm. The interaction with HIV-1 Vpr protein possibly leads its translocation to a perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 512756 Cow
- Entrez Gene: 10980 Human
- Entrez Gene: 26893 Mouse
- Entrez Gene: 100171609 Orangutan
- Entrez Gene: 100125954 Pig
- Omim: 614729 Human
- SwissProt: A6QQ21 Cow
- SwissProt: Q7L5N1 Human
see all -
別名
- COP9 (constitutive photomorphogenic), subunit 6; COP9 complex S6 antibody
- COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis) antibody
- COP9 signalosome complex subunit 6 antibody
see all
画像
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Anti-COPS6 antibody (ab77299) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 20 minutes -
ICC/IF image of ab77299 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77299, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293 and HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab77299 は 3 報の論文で使用されています。
- Liang E et al. COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein. Neuropsychiatr Dis Treat 17:1217-1227 (2021). PubMed: 33911869
- Xiao M et al. MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma. Clin Transl Med 10:e241 (2020). PubMed: 33377647
- Deng Q et al. CSN6 and Rab34 Are Involved in Androgen Receptor Trafficking in Mouse Testicular Sertoli Cells. Cell Physiol Biochem 47:2360-2368 (2018). PubMed: 29991022