製品の概要

  • 製品名Anti-COP1 antibody
    COP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to COP1
  • アプリケーション適用あり: WB, ICC/IF, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment: CLRSFKGHIN EKNFVGLASN GDYIACGSEN NSLYLYYKGL SKTLLTFKFD TVKSVLDKDR KEDDTNEFVS AVCWRALPDG ESNVLIAANS QGTIKVLELV , corresponding to amino acids 632-732 of Human COP1

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 80 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
  • 組織特異性Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the COP1 family.
    Contains 1 RING-type zinc finger.
    Contains 7 WD repeats.
  • ドメインThe RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).
  • 細胞内局在Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Constitutive photomorphogenesis protein 1 homolog antibody
    • COP antibody
    • E3 ubiquitin-protein ligase RFWD2 antibody
    • hCOP1 antibody
    • Rfwd2 antibody
    • RFWD2_HUMAN antibody
    • ring finger and WD repeat domain 2 antibody
    • RING finger and WD repeat domain protein 2 antibody
    • RING finger protein 200 antibody
    see all

Anti-COP1 antibody 画像



  • Predicted band size : 80 kDa
    COP1 antibody (ab56400) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
  • Immunofluorescence of ab56400 on NIH/3T3 cell [antibody concentration 10 ug/ml].
  • Overlay histogram showing NIH3T3 cells stained with ab56400 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56400, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

  • IHC image of COP1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56400, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-COP1 antibody (ab56400) 使用論文

This product has been referenced in:
  • Xu S  et al. TRIB2 inhibits Wnt/ß-Catenin/TCF4 signaling through its associated ubiquitin E3 ligases, ß-TrCP, COP1 and Smurf1, in liver cancer cells. FEBS Lett 588:4334-41 (2014). Read more (PubMed: 25311538) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (BEL-7402)
Gel Running Conditions Non-reduced Denaturing (10%)
Loading amount 40 µg
Treatment 80 µM Doxorubicin for 24hrs
Specification BEL-7402
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. guoqing zhu

Verified customer

投稿 Jan 22 2016

Application Western blot
Sample Rat Tissue lysate - whole (kidney)
Loading amount 20 µg
Specification kidney
Gel Running Conditions Reduced Denaturing (10-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293T)
Specification HEK293T
Fixative Formaldehyde
Permeabilization Yes - 1% triton in PBS (20 min)
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Oct 31 2012

Vielen Dank für Ihre Anfrage.

Bitte entschuldigen Sie die Verzögerung: ich hatte mich mit dem Labor, welches den Antikörper herstellt, in Verbindung gesetzt.

Hier nun die Antworten bezüglich Ihrer Fragen:

...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T cell)
Loading amount 50 µg
Specification 293T cell
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"