製品の概要

  • 製品名Anti-Connexin 45 / GJA7 antibody
    Connexin 45 / GJA7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Connexin 45 / GJA7
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Connexin 45/ GJA7.

    (Peptide available as ab91444.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Rat Lung Tissue Lysate and in the following whole cell lysates: SHSY5Y; SKNBE; SKNSH.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

Anti-Connexin 45 / GJA7 antibody 画像

  • All lanes : Anti-Connexin 45 / GJA7 antibody (ab70365) at 1 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • Anti-Connexin 45 / GJA7 antibody (ab70365) at 1 µg/ml + Lung (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa


    Exposure time : 20 minutes

Anti-Connexin 45 / GJA7 antibody (ab70365) 使用論文

ab70365 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70365.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"