Anti-Connexin 43 / GJA1 (phospho S368) 抗体 (ab30559)
Key features and details
- Rabbit polyclonal to Connexin 43 / GJA1 (phospho S368)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Connexin 43 / GJA1 (phospho S368) antibody
Connexin 43 / GJA1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Connexin 43 / GJA1 (phospho S368) -
由来種
Rabbit -
特異性
Specific for ~43k Connexin43 protein phosphorylated at Ser368. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Non human primates -
免疫原
Synthetic peptide corresponding to Rat Connexin 43/ GJA1 (phospho S368).
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ポジティブ・コントロール
- Rat hippocampal lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30559の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 42 kDa).
The immunolabeling of Connexin43 is completely eliminated by treatment with lambda phosphatase. |
特記事項 |
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WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 42 kDa). The immunolabeling of Connexin43 is completely eliminated by treatment with lambda phosphatase. |
ターゲット情報
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機能
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. -
組織特異性
Expressed in the heart and fetal cochlea. -
関連疾患
Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases. -
配列類似性
Belongs to the connexin family. Alpha-type (group II) subfamily. -
細胞内局在
Cell membrane. Cell junction > gap junction. - Information by UniProt
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参照データベース
- Entrez Gene: 2697 Human
- Entrez Gene: 14609 Mouse
- Entrez Gene: 24392 Rat
- Omim: 121014 Human
- SwissProt: P17302 Human
- SwissProt: P23242 Mouse
- SwissProt: P08050 Rat
- Unigene: 74471 Human
see all -
別名
- Connexin 43 antibody
- Connexin-43 antibody
- Cx 43 antibody
see all
画像
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All lanes : Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab30559) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate (lambda phosphatase treated)
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
The phosphospecificity of this labeling is shown in the second lane which was incubated in lambda phosphatase (1200 units for 30 min) before being exposed to GJA1.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab30559 は 13 報の論文で使用されています。
- Zhao JJ et al. The mechanisms through which auricular vagus nerve stimulation protects against cerebral ischemia/reperfusion injury. Neural Regen Res 17:594-600 (2022). PubMed: 34380899
- Lu Y et al. NF-κB and AP-1 are required for the lipopolysaccharide-induced expression of MCP-1, CXCL1, and Cx43 in cultured rat dorsal spinal cord astrocytes. Front Mol Neurosci 15:859558 (2022). PubMed: 35966011
- Huang Y et al. Glycated serum albumin decreases connexin 43 phosphorylation in the corpus cavernosum. Transl Androl Urol 11:1486-1494 (2022). PubMed: 36507487
- Zhang T et al. Propofol Mediated Protection of the Brain From Ischemia/Reperfusion Injury Through the Regulation of Microglial Connexin 43. Front Cell Dev Biol 9:637233 (2021). PubMed: 34169070
- Barnett SD et al. Novel Tocolytic Strategy: Modulating Cx43 Activity by S-Nitrosation. J Pharmacol Exp Ther 376:444-453 (2021). PubMed: 33384302