製品の概要

  • 製品名Anti-Connexin 43 / GJA1 antibody
    Connexin 43 / GJA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Connexin 43 / GJA1
  • 特異性Detects endogenous levels of total Connexin 43 / GJA1 protein.
  • アプリケーション適用あり: ELISA, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human Connexin 43/ GJA1 around the phosphorylation site of serine 261 (C-G-SP-Q-K).

  • ポジティブ・コントロール
    • Brain. HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
  • 組織特異性Expressed in the heart and fetal cochlea.
  • 関連疾患Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
    Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
    Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
    Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
    Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
  • 配列類似性Belongs to the connexin family. Alpha-type (group II) subfamily.
  • 細胞内局在Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Connexin 43 antibody
    • Connexin-43 antibody
    • Cx 43 antibody
    • Cx43 antibody
    • CXA1_HUMAN antibody
    • DFNB38 antibody
    • Gap junction 43 kDa heart protein antibody
    • Gap junction alpha-1 protein antibody
    • Gap junction protein alpha 1 43kDa (connexin 43) antibody
    • Gap junction protein alpha 1 43kDa antibody
    • Gap junction protein alpha like antibody
    • GJA 1 antibody
    • Gja1 antibody
    • GJAL antibody
    • ODD antibody
    • ODDD antibody
    • ODOD antibody
    • SDTY3 antibody
    see all

Anti-Connexin 43 / GJA1 antibody 画像

  • ab62689 at 1/50 - 1/100 dilution staining Connexin 43 / GJA1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • ab62689 at 1/500 - 1/1000 dilution staining Connexin 43 / GJA1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Anti-Connexin 43 / GJA1 antibody (ab62689) 使用論文

This product has been referenced in:
  • Fonoudi H  et al. ISL1 Protein Transduction Promotes Cardiomyocyte Differentiation from Human Embryonic Stem Cells. PLoS One 8:e55577 (2013). Flow Cyt . Read more (PubMed: 23383231) »

See 1 Publication for this product

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Merci de votre intérêt pour les anti-connexine 43 d'Abcam.

Comme expliqué par téléphone,à notre connaissanceles anticorps http://www.abcam.com/ab62689 et http://www.abcam.com/ab63851 n’ont pas en...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Testis)
Specification Testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 15 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (L/S heart muscle)
Specification L/S heart muscle
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Corneal cell line)
Specification Corneal cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 24°C
Username

Dr. Mark Carlson

Verified customer

投稿 Oct 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"