Anti-Connexin 43 / GJA1 抗体 (ab62689)
Key features and details
- Rabbit polyclonal to Connexin 43 / GJA1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Connexin 43 / GJA1 antibody
Connexin 43 / GJA1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Connexin 43 / GJA1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
非交差種: Mouse -
免疫原
Synthetic peptide corresponding to Human Connexin 43/ GJA1 (phospho S261).
Database link: P17302 -
ポジティブ・コントロール
- IHC-P: Human brain tissue. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62689の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
1/500 - 1/1000.
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IHC-P |
1/50 - 1/100.
|
特記事項 |
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ICC/IF
1/500 - 1/1000. |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. -
組織特異性
Expressed in the heart and fetal cochlea. -
関連疾患
Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases. -
配列類似性
Belongs to the connexin family. Alpha-type (group II) subfamily. -
細胞内局在
Cell membrane. Cell junction > gap junction. - Information by UniProt
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参照データベース
- Entrez Gene: 2697 Human
- Omim: 121014 Human
- SwissProt: P17302 Human
- Unigene: 74471 Human
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別名
- Connexin 43 antibody
- Connexin-43 antibody
- Cx 43 antibody
see all
画像
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ab62689 at 1/50 - 1/100 dilution staining Connexin 43 / GJA1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
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ab62689 at 1/500 - 1/1000 dilution staining Connexin 43 / GJA1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab62689 は 1 報の論文で使用されています。
- Fonoudi H et al. ISL1 Protein Transduction Promotes Cardiomyocyte Differentiation from Human Embryonic Stem Cells. PLoS One 8:e55577 (2013). Flow Cyt . PubMed: 23383231