Anti-Connexin 43 / GJA1 抗体 (ab117843)

製品の概要

  • 製品名Anti-Connexin 43 / GJA1 antibody
    Connexin 43 / GJA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Connexin 43 / GJA1
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Mouse Connexin 43/ GJA1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Mouse and Rat Heart tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab117843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

ターゲット情報

  • 機能One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
  • 組織特異性Expressed in the heart and fetal cochlea.
  • 関連疾患Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
    Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
    Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
    Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
    Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
  • 配列類似性Belongs to the connexin family. Alpha-type (group II) subfamily.
  • 細胞内局在Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Connexin 43 antibody
    • Connexin-43 antibody
    • Cx 43 antibody
    • Cx43 antibody
    • CXA1_HUMAN antibody
    • DFNB38 antibody
    • Gap junction 43 kDa heart protein antibody
    • Gap junction alpha-1 protein antibody
    • Gap junction protein alpha 1 43kDa (connexin 43) antibody
    • Gap junction protein alpha 1 43kDa antibody
    • Gap junction protein alpha like antibody
    • GJA 1 antibody
    • Gja1 antibody
    • GJAL antibody
    • ODD antibody
    • ODDD antibody
    • ODOD antibody
    • SDTY3 antibody
    see all

Anti-Connexin 43 / GJA1 antibody 画像

  • Immunohistochemical analysis of PFA-fixed paraffin-embedded rat cardiac tissue sections, labelling Conexin 43 with ab117843 at a dilution of 1/500 incubated for 12 hours at 4°C in 1% BSA in TBS. Antigen retrival was via Tris-EDTA pH 9.0 (heat mediated). Blocking was 3% BSA incubated for 1 hour at 37°C. The secondary was ab6721 at 1/500.

    See Abreview

  • IHC image of Connexin 43 / GJA1 staining in Mouse normal heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117843, 1µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Connexin 43 / GJA1 antibody (ab117843) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa


    Exposure time : 1 minute
  • IHC-Fr image of anti-Connexin 43/GJA1 staining with ab117843 on tissue sections from chicken hindbrain. The sections were blocked with 3% BSA for 1 hour at 4°C, before incubation with ab117843 (1/1000 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.

    See Abreview

Anti-Connexin 43 / GJA1 antibody (ab117843) 使用論文

ab117843 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Heart)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization No
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Mr. Helder Fonseca

Verified customer

投稿 Sep 11 2015

Application Western blot
Sample Rat Tissue lysate - whole (Bone tissue (Tibia))
Gel Running Conditions Reduced Denaturing (12,5%)
Loading amount 70 µg
Specification Bone tissue (Tibia)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Sep 08 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample Chicken Tissue sections (Hindbrain section)
Specification Hindbrain section
Permeabilization Yes - Triton X (0.1%)
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"