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Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab11370 as a replacement.
Our Abpromise guarantee covers the use of ab79010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/250.|
|WB||1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Can be blocked with Connexin 43 / GJA1 peptide (ab171366).|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||1/50 - 1/250. Use Acetone/methanol fixed tissues (1:1, 20 minutes ice cold).|
Immunohistochemistry of a human heart staining Connexin 43 / GJA1 with ab79010 at 1:500 dilution
Immunohistochemistry of a rat heart staining Connexin 43 / GJA1 with ab79010 at 1:500 dilution
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"