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Synthetic peptide corresponding to Human Connexin 40/ GJA5 aa 102-134 (N terminal).
Database link: P36382
Our Abpromise guarantee covers the use of ab38580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Connexin 40 / GJA5 with ab38580. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.
ab38580 staining Connexin 40 / GJA5 in Mouse myocyte cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in serum) for 18 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.