製品の概要

  • 製品名Anti-Connexin 37 / GJA4 antibody
    Connexin 37 / GJA4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Connexin 37 / GJA4
  • アプリケーション適用あり: IP, ELISA, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    a region within synthetic peptide: SHIRYWVLQF LFVSTPTLIY LGHVIYLSRR EERLRQKEGE LRALPSKDLH, corresponding to N terminal amino acids 73-122 of Mouse Connexin 37/ GJA4

  • ポジティブ・コントロール
    • NIH/3T3 membrane lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay, 1:312500.

WB Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • 組織特異性Expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium.
  • 配列類似性Belongs to the connexin family. Alpha-type (group II) subfamily.
  • 細胞内局在Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Connexin 37 antibody
    • Connexin-37 antibody
    • Cx37 antibody
    • CXA4_HUMAN antibody
    • Gap junction alpha 4 protein antibody
    • Gap junction alpha-4 protein antibody
    • gap junction protein, alpha 4 37kDa (connexin 37) antibody
    • gap junction protein, alpha 4, 37kD (connexin 37) antibody
    • Gap junction protein, alpha 4, 37kDa antibody
    • Gja4 antibody
    • OTTHUMP00000004181 antibody
    • OTTHUMP00000004182 antibody
    see all

Anti-Connexin 37 / GJA4 antibody 画像

  • Connexin 37 / GJA4 was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to Connexin 37 / GJA4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58918.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 45kDa; Connexin 37 / GJA4

  • Anti-Connexin 37 / GJA4 antibody (ab58918) at 10 µg/ml + NIH/3T3 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

Anti-Connexin 37 / GJA4 antibody (ab58918) 使用論文

This product has been referenced in:
  • Yang Y  et al. The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone. Biomed Res Int 2015:397264 (2015). IHC (PFA fixed) ; Mouse . Read more (PubMed: 26539488) »
  • González-Andrades M  et al. Sequential development of intercellular junctions in bioengineered human corneas. J Tissue Eng Regen Med 3:442-9 (2009). Read more (PubMed: 19415764) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab58918.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"