製品の概要

  • 製品名
    Anti-Connexin 36 / GJA9 antibody
    Connexin 36 / GJA9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Connexin 36 / GJA9
  • 特異性
    This antibody is specific for Connexin 36 / GJA9
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Dog, Chimpanzee
  • 免疫原

    A region within synthetic peptide: QNAIVNGVLQ NTENTSKETE PDCLEVKELT PHPSGLRTAS KSKLRRQEGI, corresponding to internal sequence amino acids 146-195 of Human Connexin 36/ GJA9

  • ポジティブ・コントロール
    • Fetal skeletal muscle lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab48814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-Connexin 36 / GJA9 antibody (ab48814) at 1.25 µg/ml + Fetal skeletal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Farnsworth NL  et al. Low Level Pro-inflammatory Cytokines Decrease Connexin36 Gap Junction Coupling in Mouse and Human Islets through Nitric Oxide-mediated Protein Kinase Cd. J Biol Chem 291:3184-96 (2016). Read more (PubMed: 26668311) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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