製品の概要

  • 製品名
    Anti-Connexin 30.3 / GJB4 antibody
    Connexin 30.3 / GJB4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Connexin 30.3 / GJB4
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: GPNAPSLYDN LSKKRGGLWW TYLLSLIFKA AVDAGFLYIF HRLYKDYDMP, corresponding to internal sequence amino acids 110-159 of Human Connexin 30.3/ GJB4

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/1.5625e+006.

ターゲット情報

  • 関連性
    Connexins are homologous four transmembrane domain proteins and major components of gap junctions. The GJB4 gene encodes connexin 30.3 (Cx30.3). A mutation in connexin 30.3 is causally involved in erythrokeratodermia variabilis (EKV), a mostly autosomal dominant disorder of keratinization.
  • 細胞内局在
    Plasma membrane
  • 参照データベース
  • 別名
    • Connexin 30.3 antibody
    • Cx30.3 antibody
    • Gap junction beta 4 protein antibody

画像

  • Anti-Connexin 30.3 / GJB4 antibody (ab32598) at 1.25 µg/ml + Jurkat cell lysate at 1 mg/ml

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab32598 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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