Using catecholamine-Glutaraldehyde-BSA conjugates, antibody specificity was performed with an ELISA test by competition experiments, as follows:
(a) unconjugated or conjugated catecholamine concentration at half displacement
(b) Non-reduced conjugate
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry: 1/2000 - 1/5000. Tested using a PAP techique on free floating rat locus coeruleus.
ELISA: assay dependent.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
Noradrenaline (NA) is a catecholamine neurotransmitter at postganglionic sympathetic fibres, produced following a series of steps from the essential amino acid phenylalanine. Terminal postganglionic sympathetic fibres have varicosities (where NA is synthesised and stored) which form synapses with the effector organ. The adrenal medulla is a major source of catecholamines (such as NA and adrenaline), however, most of the NA produced in the adrenal medulla is converted into adrenaline. NA is key in the fight-or-flight response and also directly increases heart rate, energy release from fat, and muscle readiness.