製品の概要

  • 製品名Anti-COMT antibody [EPR6491(B)]
    COMT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6491(B)] to COMT
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human COMT.

  • ポジティブ・コントロール
    • Fetal Liver, A431 and MOLT4 cell lysates; A431 cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
    • 組織特異性Brain, liver, placenta, lymphocytes and erythrocytes.
    • 配列類似性Belongs to the mammalian catechol-O-methyltransferase family.
    • 翻訳後修飾The N-terminus is blocked.
    • 細胞内局在Cytoplasm and Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Catechol O methyltransferase antibody
      • Catechol O-methyltransferase antibody
      • COMT antibody
      • COMT_HUMAN antibody
      • EC 2.1.1.6 antibody
      see all

    Anti-COMT antibody [EPR6491(B)] 画像



    • Predicted band size : 30 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: COMT knockout HAP1 whole cell lysate (20 µg)
      Lane 3: A431 whole cell lysate (20 µg)
      Lane 4: MOLT4 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab124813 was shown to recognize COMT when COMT knockout samples were used, along with additional cross-reactive bands. Wild-type and COMT knockout samples were subjected to SDS-PAGE.  Ab124813 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution

      Lane 1 : Fetal Liver lysate
      Lane 2 : A431 cell lysate
      Lane 3 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique

      Predicted band size : 30 kDa
    • ab124813, at 1/10 dilution staining COMT in A431 cells, (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-COMT antibody [EPR6491(B)] (ab124813) 使用論文

    ab124813 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab124813.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"