Complex IV Rodent Enzyme Activity Microplate Assay Kit (ab109911)

製品の概要

  • 製品名Complex IV Rodent Enzyme Activity Microplate Assay Kit
    Complex IV キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture extracts, Tissue
  • アッセイタイプQuantitative
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse, Rat
  • 製品の概要

    Complex IV Rodent Enzyme Activity Microplate Assay Kit (ab109911) is used to determine the activity of cytochrome c oxidase in a mouse sample with speed and simplicity. The COX enzyme is immunocaptured within the wells of the microplate and activity is determined colorimetrically by following the oxidation of reduced cytochrome c by the absorbance change at 550 nm. Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

     

     

  • 特記事項

    Buffer, detergent, and microplate should be stored at 4°C, Reagent C (reduced cytochrome c) should be stored at -20°C.

  • アプリケーション適用あり: Functional Studiesmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 別名
    • COX
    • Cytochrome c oxidase
    • Cytochrome oxidase

アプリケーション

Our Abpromise guarantee covers the use of ab109911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Complex IV Rodent Enzyme Activity Microplate Assay Kit 画像

  • Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states. Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, substrate is added, and enzyme activity is analyzed by measuring the change in absorbance of either the substrate or the product of the reaction (depending upon which enzyme is being analyzed). By analyzing the enzyme's activity in an isolated context, outside of the cell and free from any other variables, an accurate measurement of the enzyme's functional state can be understood.
  • Figure 1. To determine the activity in the sample, calculate the slope by using microplate software or by manual calculations using one of the two methods shown. Compare the sample rate with the rate of the control (normal) sample and with the rate of the null (background) to get the relative Complex IV activity. (A)The rate is determined by calculating the gradient of the initial slope over the linear region. (B) The rate is determined by calculating the slope between two points within the linear region.

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Complex IV Rodent Enzyme Activity Microplate Assay Kit (ab109911) 使用論文

This product has been referenced in:
  • Li W  et al. Dysfunction of mitochondria and deformed gap junctions in the heart of IL-18-deficient mice. Am J Physiol Heart Circ Physiol 311:H313-25 (2016). Read more (PubMed: 27288439) »
  • Yao X  et al. Mitochondrial ROS Induces Cardiac Inflammation via a Pathway through mtDNA Damage in a Pneumonia-Related Sepsis Model. PLoS One 10:e0139416 (2015). Functional Studies ; Rat . Read more (PubMed: 26448624) »

See all 20 Publications for this product

Product Wall


1) Yes, the mitochondrial isoaltion kit is suitable for use with all kits listed.

2) Suspend the prepared mitochondria to 5.5 mg/mL as described in the attached document and follow from step 5 (adding detergent to mitochondia) to generat...

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The protocol makes specific reference to mitochondria and tissue extracts. When it comes to cells, there is a significant loss of protein in the pellet after extraction as you mentioned on the phone. I would recommend either re-measuring the concentr...

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We provide the cytochrome c in reduced form for this kit.
I can say the protein is active and also the detergent is mild. So if cytochrome c functions as a dimer then you can anticipate this conformation in the assay solution.
We are unsure wh...

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30ug Lung mitochondrion samples from mice in buffer containing 250 mM sucrose, 20 mM HEPES, 1 mM EDTA, 1 mM EGTA, 0.25% protease inhibitor, and 0.5% BSA, pH 7.4 were used for the assay.
Username

Dr. Amit Agarwal

Verified customer

投稿 Jul 24 2013

Thank you for your technical enquiry and interest in our products.

I have discussed this with my colleagues in the Lab. This kit (ab109911: Complex IV Rodent Enzyme) measures in a standard spectrophotometer the absorbance of reduced cytochrom...

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Thank you for your interest.

The product code number for this item is ab140219. However, it is not published so the datasheet can't get accessed on the public site.

I would advise to place an order via e-mail: mailto:orders@abcam.co...

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Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you.

The three kits mentioned (ab109721, ab109911 and ab109908) use 10% lauryl maltoside detergent to prepare the sample at 5 mg/mL. The...

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Thank you for your contact.

I have discussed your enquiry with my colleagues and can confirm that Reagent C is available for special request. The sale price is USD50.00 or GBP40.00 /unit. We currently have this item in stock.

Could ...

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Thank you for your enquiry.


I can confirm that In each of these kits we use a non-ionic native detergent at an optimized concentration to maintain OXPHOS complex assembly and activity. This detergent is 1% final N-dodecyl-b-D-maltoside ...

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Thank you for your enquiry which has been forwarded to me as my colleague, who you spoke to on the phone, is away from the office today.

We would usually recommend to purchase RHM homogenate (ab110347) as positive control sample. I can recom...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"