Complex IV Immunocapture Kit (ab109801)
Key features and details
- Assay type: Quantitative
製品の概要
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製品名
Complex IV Immunocapture Kit
Complex IV キット 製品一覧 -
アッセイタイプ
Quantitative -
種交差性
交差種: Cow, Human -
製品の概要
ab109801 Complex IV Immunocapture Kit allows isolation of the cytochrome c oxidase complex from small amounts of tissue.
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.
This kit facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of Complex IV. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109801 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
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特記事項
Related products
Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 250 µg Monoclonal Complex IV antibody irreversibly crosslinked to protein G-agarose beads 1 x 250µg -
研究分野
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別名
- cco
- COX
- Cytochrome c oxidase
- Cytochrome oxidase
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参照データベース
- Entrez Gene: 4512 Human
- Entrez Gene: 4513 Human
- Entrez Gene: 4514 Human
- Entrez Gene: 1345 Human
- Entrez Gene: 1351 Human
- Entrez Gene: 1329 Human
- Entrez Gene: 1349 Human
- Entrez Gene: 1337 Human
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関連製品
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Related Products
画像
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Complex IV immunoprecipitation using antibody ab109863/MS101c crosslinked to protein G-agarose beads as product ab109801
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab109801 は 11 報の論文で使用されています。
- Thiriveedi VR et al. Glutathionylated and Fe-S cluster containing hMIA40 (CHCHD4) regulates ROS and mitochondrial complex III and IV activities of the electron transport chain. Redox Biol 37:101725 (2020). PubMed: 32971361
- Wright JN et al. Acute increases in O-GlcNAc indirectly impair mitochondrial bioenergetics through dysregulation of LonP1-mediated mitochondrial protein complex turnover. Am J Physiol Cell Physiol 316:C862-C875 (2019). PubMed: 30865517
- Morscher RJ et al. Mitochondrial translation requires folate-dependent tRNA methylation. Nature 554:128-132 (2018). PubMed: 29364879
- Oliva CR et al. Identification of Small Molecule Inhibitors of Human Cytochrome c Oxidase That Target Chemoresistant Glioma Cells. J Biol Chem 291:24188-24199 (2016). IP . PubMed: 27679486
- Desmurs M et al. C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization. Mol Cell Biol 35:1139-56 (2015). PubMed: 25605331