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The Complex IV Human Enzyme Activity Microplate Assay Kit is used to determine the activity of cytochrome c oxidase in a human sample with greater speed and simplicity. The COX enzyme is immunocaptured within the wells of the microplate and activity is determined colorimetrically by following the oxidation of reduced cytochrome c by the absorbance change at 550 nm. Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
Store Tube 1, detergent, and microplate at 4°C – DO NOT FREEZE. Store Reagent C at -20°C or at -80°C for long-term storage.
|96-well microplate (12 strips)||1 unit|
|Buffer||1 x 10ml|
|Detergent||1 x 1ml|
|Reagent C (Cytochrome c)||1 x 1ml|
Our Abpromise guarantee covers the use of ab109909 in the following tested applications.
|Functional Studies||Use at an assay dependent dilution.|