Complex IV Human Enzyme Activity Dipstick Assay Kit (ab109876)
製品の概要
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製品名
Complex IV Human Enzyme Activity Dipstick Assay Kit
Complex IV キット 製品一覧 -
サンプルの種類
Cell culture extracts, Tissue -
アッセイタイプ
Sandwich (quantitative) -
種交差性
交差種: Cow, Human
非交差種: Mouse, Rat -
製品の概要
Contains 30 or 90 dipsticks and necessary components to quantify the activity of the cytochrome c oxidase enzyme complex from human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.
The isolation of mitochondria is not necessary for the performance of this assay. In this kit the specificity of anti-COX monoclonal antibodies (mAbs) is combined with traditional methods for determining COX enzyme activity by histochemical methods and in-gel activity assays. First, the COX enzyme complex is immnocaptured (i.e immunoprecipated in active form) on the dipstick. Second, the dipstick is immersed in COX activity buffer containing reduced cytochrome c and di-amino benzidinetetrachloride (DAB), which serves as the reporter of COX activity. Immunocaptured COX oxidizes cytochrome c, which then oxidizes DAB to form a red-colored precipitate at the COX antibody line on the dipstick. In addition to being quick, the reaction is cyanide-sensitive. The signal intensity of this precipitate corresponds to the level of COX activity in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system. -
特記事項
Store dipsticks at room temperature in their provided container and out of direct sunlight. High humidity conditions should be avoided.
Store Buffer A, B, and C at 4°C or at -20°C for long term storage.
Store Tubes 1 and 2 at -80°C; they can also be aliquoted upon receipt to prevent freeze/thaw cycles.
Tube 3 can be stored at room temperature.
Range of complex IV / cytochrome c oxidase assay kits
Biochemical assay - ab239711
Immunocapture with biochemical assay (plate-based) - ab109911 (rodent) and ab109909 (human)
Immunocapture with biochemical assay (dipstick) - ab109878 (rodent) and ab109876 (human) (this kit)
Immunocapture with biochemical assay and ELISA - ab109910 (human)
ELISA - ab179880 (human)
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アプリケーション
適用あり: Functional Studiesmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Please refer to protocols. -
内容 30 tests 96-well microplate 2 units Buffer B (10X Blocking solution) 2 x 1ml Dipsticks 1 x 30 units Extraction Buffer (ab260490) 1 x 15ml Tube 1 (DAB 100X stock) 1 x 100µl Tube 2 (reduced cytochrome c 20X stock) 1 x 500µl Tube 3 (Buffer for activity assay) 1 x 10ml Wash buffer 1 x 1.5ml -
研究分野
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別名
- cco
- COX
- Cytochrome c oxidase
- Cytochrome oxidase
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参照データベース
- Entrez Gene: 4512 Human
- Entrez Gene: 4513 Human
- Entrez Gene: 4514 Human
- Entrez Gene: 1345 Human
- Entrez Gene: 1351 Human
- Entrez Gene: 1329 Human
- Entrez Gene: 1349 Human
- Entrez Gene: 1337 Human
see all
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109876の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent dilution.
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特記事項 |
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Functional Studies
Use at an assay dependent dilution. |
画像
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Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. Dipstick ELISA Kits extend this concept by utilizing the well-established lateral flow concept, wherein capture antibodies are striped onto nitrocellulose membrane and a wicking pad draws the sample through the antibody bands. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.
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Figure 1. An example using ab109876 to measure Complex IV activity in fibroblast protein extracts. Developed dipsticks from a 1:2 dilution series using a positive control sample and the associated standard curve. Starting material was 100 µg of fibroblast protein extract.
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Figure 2. An example using ab109876 to measure Complex IV activity in fibroblast protein extracts. Based on the standard curve, 50 µg of protein extract were loaded onto a dipstick for each sample. The figure shows four developed dipsticks, a control sample (1) and four unknowns (2-6). The analysis of the signal intensity and interpolation from the standard curve showed that the unknown samples have between 15-61% of normal Complex IV activity levels.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab109876 は 15 報の論文で使用されています。
- Torraco A et al. A novel homozygous variant in COX5A causes an attenuated phenotype with failure to thrive, lactic acidosis, hypoglycemia, and short stature. Clin Genet 102:56-60 (2022). PubMed: 35246835
- Suárez-Rivero JM et al. UPRmt activation improves pathological alterations in cellular models of mitochondrial diseases. Orphanet J Rare Dis 17:204 (2022). PubMed: 35581596
- Durhuus JA et al. Simvastatin improves mitochondrial respiration in peripheral blood cells. Sci Rep 10:17012 (2020). PubMed: 33046789
- Zhao T et al. PISD is a mitochondrial disease gene causing skeletal dysplasia, cataracts, and white matter changes. Life Sci Alliance 2:N/A (2019). PubMed: 30858161
- Nimmo GAM et al. Bi-allelic mutations of LONP1 encoding the mitochondrial LonP1 protease cause pyruvate dehydrogenase deficiency and profound neurodegeneration with progressive cerebellar atrophy. Hum Mol Genet 28:290-306 (2019). PubMed: 30304514