Anti-Complex III Immunocapture 抗体 [11A51H12] (ab109862)

製品の概要

  • 製品名
    Anti-Complex III Immunocapture antibody [11A51H12]
  • 製品の詳細
    Mouse monoclonal [11A51H12] to Complex III Immunocapture
  • 特異性
    100 µg ab109862 can capture at least 10 µg Complex III from 1 mg solubilized bovine heart mitochondria.
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Bovine Heart Complex III

  • ポジティブ・コントロール
    • Bovine heart and Human mitochondria
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. PubMed: 22523357
IP Use at 10-1 µg/mg of lysate.

ターゲット情報

  • 関連性
    Complex III, or ubiquinol cytochrome c oxidoreductase, is a complex of 11 subunits and catalyses electron transfer from reduced ubiquinone or coenzyme Q 10 to cytochrome c. The complex contains 11 different subunits, one of which, cytochrome b, is encoded on mitochondrial DNA, while the remainder subunits are nuclear-encoded. Genetic complex III deficiencies occur but are rare.

画像

  • Staining of Complex III Lane 1: 0.1mg of Bovine mitochondria Lane 2: 0.05mg Bovine mitochondria Lane 3: 0.05mg Human mitochondria Immunoprecipitated using ab109862 at 10 µg/mg lysate.

参考文献

This product has been referenced in:
  • Beutner G  et al. Initiation of electron transport chain activity in the embryonic heart coincides with the activation of mitochondrial complex 1 and the formation of supercomplexes. PLoS One 9:e113330 (2014). IP ; Mouse . Read more (PubMed: 25427064) »
  • Jonckheere AI  et al. A complex V ATP5A1 defect causes fatal neonatal mitochondrial encephalopathy. Brain 136:1544-54 (2013). Read more (PubMed: 23599390) »

See all 5 Publications for this product

レビューと Q&A

Thank you for contacting us. Please click these links to find the suitable antibodies. Type the MS code in Abcam search box to get the Abcam catalogue number. Complex I http://www.mitosciences.com/complex_i.html Complex IV http://www.mi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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