製品の概要

  • 製品名
    Complex II WB Antibody Cocktail
    Complex II キット 製品一覧
  • アッセイタイプ
    Quantitative
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 製品の概要

    Contains a total of 305 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).

    Cocktail Antibodies:

    Mouse C-V-alpha [15H4C4] monoclonal:

    Amount: 50 µg

    Working concentration: 1 µg/ml

    Mouse C-II-30 (FeS) [21A11AE7] monoclonal:

    Amount: 250 µg

    Working concentration: 5 µg/ml

    Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:

    Amount: 5 µg

    Working concentration: 0.1 µg/ml

  • アプリケーション
    適用あり: WBmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 6 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting.

画像

  • All lanes : Complex II WB Antibody Cocktail (ab110410) at 1/250 dilution

    Lane 1 : human heart mitochondria at 10 µg
    Lane 2 : Immunoprecipitated Complex II at 0.5 µg


    The Western blot cocktail ab110410 contains two Complex II subunits SDHA (70 kDa) and SDHB (30 kDa)and a general mitochondrial marker ATP synthase alpha subunit (F1α, 55 kDa). All three are present in the mitochondrial sample (1). The Complex II sample purified by Complex II immunoprecipitation beads (ab109799) shows only Complex II subunits (2).

参考文献

This product has been referenced in:
  • Yan H  et al. NR4A1-induced increase in the sensitivity of a human gastric cancer line to TNFa-mediated apoptosis is associated with the inhibition of JNK/Parkin-dependent mitophagy. Int J Oncol 52:367-378 (2018). Read more (PubMed: 29207128) »
  • Guerra F  et al. Placing mitochondrial DNA mutations within the progression model of type I endometrial carcinoma. Hum Mol Genet 20:2394-405 (2011). WB ; Human . Read more (PubMed: 21470976) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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