Anti-Complex II Immunocapture 抗体 [4H12BG12AG2] (ab109865)

製品の概要

  • 製品名
    Anti-Complex II Immunocapture antibody [4H12BG12AG2]
  • 製品の詳細
    Mouse monoclonal [4H12BG12AG2] to Complex II Immunocapture
  • 由来種
    Mouse
  • 特異性
    Human, bovine, mouse
  • アプリケーション
    適用あり: ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Cow, Human
  • 免疫原

    Bovine Heart Complex II, with traces of Complex III.

  • ポジティブ・コントロール
    • Bovine heart and Human mitochondria. IF/ICC: HeLa cell line
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
IP Use at 100-1 µg/mg of lysate.

ターゲット情報

  • 関連性
    Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
  • 細胞内局在
    Mitochondrial

画像

  • ICC/IF image of ab109865 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109865, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Complex II immunoprecipitation using antibody ab109865  crosslinked to protein G-agarose beads.  Complex II was immunoprecipitated from a lauryl maltoside detergent extract of 1000 ug bovine heart mitochondria in lane 1 and 500 ug human heart mitochondria in lane 2.  The gel was stained with coomassie brilliant blue.

参考文献

This product has been referenced in:

See all 6 Publications for this product

レビューと Q&A

This antibody has been used in western blot in the following publication:

http://jn.nutrition.org/cgi/pmidlookup?view=long&pmid=21346097

We do fully guarantee the antibody to work in WB however, we feel that a better option for ...

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Thank you for contacting us. Please click these links to find the suitable antibodies. Type the MS code in Abcam search box to get the Abcam catalogue number. Complex I http://www.mitosciences.com/complex_i.html Complex IV http://www.mi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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