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Complex II Enzyme Activity Microplate Assay Kit is designed for determining the Complex II activity in a human, mouse, rat or bovine sample. Each of the 96 wells in the kit has been coated with an anti-Complex II monoclonal antibody (mAb) which purifies the enzyme from a complex sample such as mitochondria, tissue homogenate or cell lysate. After this in-well purification the production of ubiquinol by the enzyme is coupled to the reduction of the dye DCPIP (2,6-diclorophenolindophenol) and a decreases in its absorbance at 600 nm, which in turn recycles the substrate ubiquinone.
Succinate, Ubiquinone 2, DCPIP and Lipid?Phospholipd Mix should be stored at -80°C. All other components should be stored at 4°C.
|10X Blocking Solution||1 x 5ml|
|10X Detergent||2 x 1ml|
|20X Buffer||1 x 15ml|
|Complex II Activity Buffer||1 x 25ml|
|DCPIP||1 x 250µl|
|Phospholipid||1 x 6ml|
|Pre-coated 96-well microplate (12 strips)||1 unit|
|Succinate||1 x 500µl|
|Ubiquinone 2||1 x 60µl|
Our Abpromise guarantee covers the use of ab109908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|