製品の概要

  • 製品名Anti-COMP antibody
    COMP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to COMP
  • アプリケーション適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human COMP.

    (Peptide available as ab74523.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in mouse cartilage, human skeletal muscle and human heart tissue lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab74524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 21939397

ターゲット情報

  • 機能May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
  • 組織特異性Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect.
  • 関連疾患Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1) [MIM:132400]. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types.
    Defects in COMP are the cause of pseudoachondroplasia (PSACH) [MIM:177170]. PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood.
  • 配列類似性Belongs to the thrombospondin family.
    Contains 4 EGF-like domains.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 8 TSP type-3 repeats.
  • 発生段階Present during the earliest stages of limb maturation and is later found in regions where the joints develop.
  • ドメインThe cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.
    The TSP C-terminal domain mediates interaction with FN1 and ACAN.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) antibody
    • Cartilage oligomeric matrix protein antibody
    • Cartilage oligomeric matrix protein precursor antibody
    • COMP antibody
    • COMP_HUMAN antibody
    • EDM 1 antibody
    • EDM1 antibody
    • EPD 1 antibody
    • EPD1 antibody
    • Epiphyseal dysplasia 1 antibody
    • Epiphyseal dysplasia 1 multiple antibody
    • Epiphyseal dysplasia multiple 1 antibody
    • MED antibody
    • MGC13181 antibody
    • MGC149768 antibody
    • PSACH antibody
    • pseudoachondroplasia (epiphyseal dysplasia 1, multiple) antibody
    • Pseudoachondroplasia antibody
    • THBS 5 antibody
    • THBS5 antibody
    • Thrombospondin 5 antibody
    • Thrombospondin-5 antibody
    • Thrombospondin5 antibody
    • TSP5 antibody
    see all

Anti-COMP antibody 画像

  • All lanes : Anti-COMP antibody (ab74524) at 1 µg/ml

    Lane 1 : Mouse Cartilage Tissue Lysate
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 82 kDa
    Observed band size : 80,100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds

Anti-COMP antibody (ab74524) 使用論文

This product has been referenced in:
  • Liu CF  et al. Spatial and temporal expression of molecular markers and cell signals during normal development of the mouse patellar tendon. Tissue Eng Part A : (2011). IHC-Fr ; Mouse . Read more (PubMed: 21939397) »
  • Crovace A  et al. Histological and immunohistochemical evaluation of autologous cultured bone marrow mesenchymal stem cells and bone marrow mononucleated cells in collagenase-induced tendinitis of equine superficial digital flexor tendon. Vet Med Int 2010:250978 (2010). Read more (PubMed: 20445779) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (cartilage)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K in 0.05 M Tris-HCl, pH 7,6
Permeabilization No
Specification cartilage
Fixative Formaldehyde
Username

Marjan Steppe

Verified customer

投稿 Dec 07 2016

Thank you for contacting us.

The antibody will most likely be okay. If you have some samples that aren't too precious, you may want to try a WB or even dot blot to see if you get reactivity. Storage at 4C is not ideal, but the antibody may n...

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Thank you for contacting Abcam.

The anti-COMP antibody, ab74524 has not been tested in immunofluoresce. If we have tested an antibody in a particular application and it failed to give any signal, then we will put that information on the datash...

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Thank you for your reply.

As ab42256 is specific peptide, there is no alternative for it. Also, with this product there is currently a 1-2 week wait until we are able to send it out. With this product, we only begin the restocking process once...

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Thank you for contacting Abcam.

Please find a list of possible alternatives below:

ab7973 alternative
http://www.abcam.com/Bcl2-antibody-ab18210.html
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http://www.abcam.com/Bcl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"