製品の概要

  • 製品名Anti-Collagen X antibody
    Collagen X 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Collagen X
  • 特異性ab58632 recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen.
  • アプリケーション適用あり: ICC/IF, WB, IP, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Cow, Human
  • 免疫原

    Full length native protein (purified) Type X collagen from rat chondrosarcoma cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: Whole serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/100 - 1/300.
IP 1/20 - 1/40.
IHC-Fr Use at an assay dependent concentration. Enzymatic or heat (41°C) pretreatment is recommended.

ターゲット情報

  • 機能Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
  • 関連疾患Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.
  • 配列類似性Contains 1 C1q domain.
  • 翻訳後修飾Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • COAA1_HUMAN antibody
    • Col10a 1 antibody
    • COL10A1 antibody
    • Collagen alpha 1(X) chain antibody
    • Collagen alpha-1(X) chain antibody
    • Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) antibody
    • Collagen type X alpha 1 antibody
    • Collagen X alpha 1 polypeptide antibody
    • CollagenX antibody
    • fa66d11 antibody
    • fb10c08 antibody
    • OTTHUMP00000040411 antibody
    • Procollagen type X alpha 1 antibody
    • Schmid metaphyseal chondrodysplasia antibody
    • wu:fa66d11 antibody
    • wu:fb10c08 antibody
    see all

Anti-Collagen X antibody 画像

  • Immunofluorescence analysis of mouse bone marrow stromal cells, staining Collagen X with ab58632.

    Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 before incubating with primary antibody (1/50) overnight. An AlexaFluor®488-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody.
  • Anti-Collagen X antibody (ab58632) at 1/500 dilution + HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 66 + 64 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,50 kDa,98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds
    The band observed at 64 kDa could potentially be a cleaved form of Collagen X due to the presence of a 18 amino acid signal peptide.

Anti-Collagen X antibody (ab58632) 使用論文

This product has been referenced in:
  • Bian Q  et al. Excessive Activation of TGFß by Spinal Instability Causes Vertebral Endplate Sclerosis. Sci Rep 6:27093 (2016). IHC-P ; Mouse . Read more (PubMed: 27256073) »
  • Xu X  et al. Aberrant Activation of TGF-ß in Subchondral Bone at the Onset of Rheumatoid Arthritis Joint Destruction. J Bone Miner Res 30:2033-43 (2015). IHC . Read more (PubMed: 25967237) »

See all 28 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (cartilage)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteinase K 10ug/ml
Permeabilization No
Specification cartilage
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 24°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 16 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Bone Marrow)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Specification Bone Marrow
Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative 10% NBF
Username

Ms. Premier Laboratory

Verified customer

投稿 May 04 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Knee)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Specification Knee
Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative 10%NBF fix, 10% Formic Acid decalcification
Username

Ms. Premier Laboratory

Verified customer

投稿 May 04 2016

Antibody ab58632 is our recommendation, given the number of references that have used it for IHC of FFPE tissue. Here is a link to the list of references, annotated "IHC-P" for paraffin.

http://www.abcam.com/collagen-x-antibody-ab58632-referen...

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Thank you for contacting us. The anti-Collagen X antibody ab58632 is a whole serum antibody and the concentration has not been determined. Generally concentrations are only relevant for purified antibodies, since products from ascites or whole serum ma...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for contacting us and for your interest in our products.

I am sorry to confirm that as far as we are aware, ab58632 has never been tested with samples from sheep. All tested and guaranteedspecies cross-reactivity information is sta...

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Thank you for contacting us.

The antibody is whole, unpurified antiserum, and the antibody concentration is not determined. Dilutions for some common applications are suggested on the datasheet. The appropriate isotype control for this antib...

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Thank you for your inquiry. Please find a list of possible positive controls below: ab2426: skin, lymph nodes Click here (or use the following: http://www.abcam.com/index.html?datasheet=2426). ab3773: cartilage samples Click here (or use the followin...

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Thank you for your call yesterday and for your patience while I have been in touch with the lab. For ab58632, we recommend using the antibody at 1:50-1:100 and incubating overnight. The sections were treated with 0.25% trypsin in 1 mM EDTA for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"