Anti-Collagen VII 抗体 (ab93350)


  • 製品名Anti-Collagen VII antibody
    Collagen VII 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Collagen VII
  • アプリケーション適用あり: WB, IP, ELISA, Flow Cyt, Enzyme Immunoassay, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human, Marmoset (common)
  • 免疫原

    Full length native protein (purified) corresponding to Human Collagen VII.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab93350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
Enzyme Immunoassay
  • 追加情報ELISA: Use at an assay dependent dilution.
    EIA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 2.5 - 10 µg/ml.
    IP: Use at a concentration of 2.5 - 10 µg/ml.
    WB: Use at a concentration of 0.2 - 0.8 µg/ml. Predicted molecular weight: 295 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
    • 関連疾患Epidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
      Epidermolysis bullosa dystrophica, autosomal dominant
      Epidermolysis bullosa dystrophica, autosomal recessive
      Epidermolysis bullosa dystrophica, Pasini type
      Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
      Transient bullous dermolysis of the newborn
      Epidermolysis bullosa dystrophica, pretibial type
      Epidermolysis bullosa dystrophica, Bart type
      Epidermolysis bullosa pruriginosa
      Nail disorder, non-syndromic congenital, 8
      Epidermolysis bullosa dystrophica, with subcorneal cleavage
    • 配列類似性Contains 1 BPTI/Kunitz inhibitor domain.
      Contains 9 fibronectin type-III domains.
      Contains 2 VWFA domains.
    • 翻訳後修飾Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    • 細胞内局在Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alpha 1 type VII collagen antibody
      • CO7A1_HUMAN antibody
      • Col7a1 antibody
      • Collagen alpha 1(VII) chain antibody
      • Collagen alpha-1(VII) chain antibody
      • Collagen type VII alpha 1 antibody
      • Collagen VII alpha 1 polypeptide antibody
      • CollagenVII antibody
      • EBD 1 antibody
      • EBD1 antibody
      • EBDCT antibody
      • EBR 1 antibody
      • EBR1 antibody
      • LC collagen antibody
      • Long chain collagen antibody
      • Long-chain collagen antibody
      see all

    Anti-Collagen VII antibody 画像

    • Immunohistochemical staining of Collagen VII in human skin tissue sections with ab93350. Samples were fixed with formaldehyde and enzymatic antigen retrieval was performed with Proteinase K. Tissue was blocked with 1% BSA for 10 minutes at 21°C before ab93350 was added (1/250) for 2 hours at 21°C. A biotin conjugated goat anti-rabbit secondary was used at 1/250.

      See Abreview

    Anti-Collagen VII antibody (ab93350) 使用論文

    ab93350 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (skin)
    Permeabilization No
    Specification skin
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Acetone

    Abcam user community

    Verified customer

    投稿 Jul 12 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
    Sample Human Tissue sections (Skin)
    Specification Skin
    Permeabilization No
    Fixative Formaldehyde

    Mr. Carl Hobbs

    Verified customer

    投稿 Aug 19 2014

    Thank you for your inquiry.

    I heard back from the lab that our anti-Collagen VII antibody is specific only for helical domains (collagen VII fragments). The non-collagen fragments obtained after pepsin digestion are broken and cannot be detec...

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    Thank you for contacting us.
    Both antibodies were run under reducing and denaturing conditions.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use o...

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    Thank you for your enquiry.

    I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in ma...

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    Thank you for contacting us. The BLAST results show 84% homology between the full-length human (immunogen) and mouse and is likely to work. If you are interested in this product we do have a testing discount program which acts as a buy one get one free...

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