Anti-Collagen VII 抗体 (ab223639)
Key features and details
- Goat polyclonal to Collagen VII
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Collagen VII antibody
Collagen VII 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Collagen VII -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Dog, Human, Pig -
免疫原
Synthetic peptide corresponding to Human Collagen VII aa 50-150 (internal sequence) (Cysteine residue). (NP_000085.1).
Database link: Q02388 -
ポジティブ・コントロール
- WB: Rat testis lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab223639 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab223639の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 1.5 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 295 kDa).
A 1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.1 - 1.5 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 295 kDa). A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. -
関連疾患
Epidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
Epidermolysis bullosa dystrophica, autosomal dominant
Epidermolysis bullosa dystrophica, autosomal recessive
Epidermolysis bullosa dystrophica, Pasini type
Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
Transient bullous dermolysis of the newborn
Epidermolysis bullosa dystrophica, pretibial type
Epidermolysis bullosa dystrophica, Bart type
Epidermolysis bullosa pruriginosa
Nail disorder, non-syndromic congenital, 8
Epidermolysis bullosa dystrophica, with subcorneal cleavage -
配列類似性
Contains 1 BPTI/Kunitz inhibitor domain.
Contains 9 fibronectin type-III domains.
Contains 2 VWFA domains. -
翻訳後修飾
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 403467 Dog
- Entrez Gene: 1294 Human
- Entrez Gene: 12836 Mouse
- Entrez Gene: 301012 Rat
- Omim: 120120 Human
- SwissProt: Q02388 Human
- SwissProt: Q63870 Mouse
- Unigene: 476218 Human
see all -
別名
- Alpha 1 type VII collagen antibody
- CO7A1_HUMAN antibody
- Col7a1 antibody
see all
画像
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Anti-Collagen VII antibody (ab223639) at 0.5 µg/ml + Rat testis lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 295 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab223639 は 2 報の論文で使用されています。
- Cui Y et al. Clusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a1. Mol Ther Nucleic Acids 26:1336-1350 (2021). PubMed: 34853731
- Saikia P et al. Quantitative proteomic comparison of myofibroblasts derived from bone marrow and cornea. Sci Rep 10:16717 (2020). PubMed: 33028893