製品の概要
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製品名Anti-Collagen V antibody
Collagen V 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Collagen V
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由来種Rabbit
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特異性Negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
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アプリケーション適用あり: ICC, Sandwich ELISA, IHC-Fr, IP, WB, IHC-Pmore details
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種交差性交差種: Mouse, Cow, Human
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免疫原
Full length native protein (purified) corresponding to Collagen V aa 1-1745.
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ポジティブ･コントロール
- IHC :Dog skeletal muscle tissue, Human pancreas tissue.
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特記事項
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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バッファーpH: 8.00
Preservative: 0.01% Sodium azide
Constituents: 4.8% Sodium borate, 0.15% EDTA, 0.44% Sodium chloride -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab7046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | Use at an assay dependent concentration. PubMed: 18385800 | |
| Sandwich ELISA | Use a concentration of 10 µg/ml. Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582). | |
| IHC-Fr | 1/50 - 1/200. | |
| IP | 1/5000 - 1/10000. | |
| WB | 1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended. | |
| IHC-P | 1/50 - 1/200. |
ターゲット情報
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機能Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
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関連疾患Ehlers-Danlos syndrome 1
Ehlers-Danlos syndrome 2 -
配列類似性Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 laminin G-like domain. -
ドメインThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
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翻訳後修飾Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines. -
細胞内局在Secreted > extracellular space > extracellular matrix.
- Information by UniProt
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参照データベース
- Entrez Gene: 282192 Cow
- Entrez Gene: 1289 Human
- Entrez Gene: 1290 Human
- Entrez Gene: 50509 Human
- Entrez Gene: 12831 Mouse
- Entrez Gene: 12832 Mouse
- Entrez Gene: 53867 Mouse
- Omim: 120190 Human
see all -
別名
- AB collagen antibody
- Alpha 1 type V collagen antibody
- Alpha 2 type V collagen antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)This image is courtesy of an anonymous Abreview.ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody. -
Immunohistochemistry (Frozen sections) - Anti-Collagen V antibody (ab7046)This image is courtesy of an anonymous Abreview.ab7046 staining Collagen V in Dog skeletal muscle tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone. The primary antibody was diluted 1/100 in 10% Normal Goat Serum (ab7481) in PBS-T and incubated with the sample for 12 hours at 4°C. A Rhodamine-conjugated Goat anti-Rabbit IgG was used as the secondary antibody, diluted 1/200.
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Western blot - Anti-Collagen V antibody (ab7046)Anti-Collagen V antibody (ab7046) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Observed band size: 180 kDa
Additional bands at: 122 kDa, 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung (left) and placenta (right) sections labelling collagen V with ab7046 at a dilution of 1/200 for 45 minutes at room temperature. An antigen retrival step was performed with 0.01 M sodium citrate buffer pH 6.0 at 100ºC for 20 mins.
プロトコール
参考文献
This product has been referenced in:
- Steigedal TS et al. Nephronectin is Correlated with Poor Prognosis in Breast Cancer and Promotes Metastasis via its Integrin-Binding Motifs. Neoplasia 20:387-400 (2018). WB . Read more (PubMed: 29539586) »
- McKay TB et al. Acute hypoxia influences collagen and matrix metalloproteinase expression by human keratoconus cells in vitro. PLoS One 12:e0176017 (2017). WB ; Human . Read more (PubMed: 28426715) »
レビューと Q&A
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