製品の概要

  • 製品名Anti-Collagen V antibody
    Collagen V 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Collagen V
  • 特異性This antibody recognises Collagen Type V and it has negligible cross-reactivity with Type I,II, III, IV, or VI collagens. Non-specific cross reaction with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • アプリケーション適用あり: WB, IP, IHC-Fr, ELISAmore details
  • 種交差性
    交差種: Cow, Human
    交差が予測される動物種: all Mammals
  • 免疫原

    Collagen Type V from Human and Bovine placenta.

  • 特記事項Antibody concentration is determined by UV absorbance at 280 nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 0.125M Sodium borate, 0.075M Sodium chloride, 0.005M EDTA. pH 8.0
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab34714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
IHC-Fr
ELISA
  • 追加情報ELISA: 1/4000 - 1/8000.
    IHC-Fr: 1/50 - 1/200.
    IP: Use at an assay dependent dilution.
    WB: 1/5000 - 1/10,000. Predicted molecular weight: 180 kDa.

    Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
    • 関連疾患Ehlers-Danlos syndrome 1
      Ehlers-Danlos syndrome 2
    • 配列類似性Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 laminin G-like domain.
    • ドメインThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
    • 翻訳後修飾Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Sulfated on 40% of tyrosines.
    • 細胞内局在Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AB collagen antibody
      • Alpha 1 type V collagen antibody
      • Alpha 2 type V collagen antibody
      • CO5A1_HUMAN antibody
      • COL5A1 antibody
      • Col5A2 antibody
      • COL5A2 protein antibody
      • Col5A3 antibody
      • Collagen alpha 1(V) chain antibody
      • Collagen alpha 2 (V) chain precursor antibody
      • Collagen alpha 2(V) chain antibody
      • Collagen alpha 3(V) chain antibody
      • Collagen alpha-1(V) chain antibody
      • Collagen fetal membrane A polypeptide antibody
      • Collagen type V alpha 1 antibody
      • Collagen type V alpha 2 antibody
      • Collagen type V alpha 3 antibody
      • Collagen V alpha 2 polypeptide antibody
      • CollagenV antibody
      • MGC105115 antibody
      • OTTHUMP00000064637 antibody
      • Pro alpha 1 type V collagen antibody
      • Pro alpha 3(V) collagen antibody
      • Procollagen alpha 2(V) antibody
      • Type V preprocollagen alpha 2 chain antibody
      see all

    Anti-Collagen V antibody (ab34714) 使用論文

    ab34714 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab34714.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"