Anti-Collagen Type V 抗体 [1E2-E4/Col5] (ab112551)

製品の概要

  • 製品名Anti-Collagen Type V antibody [1E2-E4/Col5]
    Collagen Type V 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1E2-E4/Col5] to Collagen Type V
  • 特異性ab112551 has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
  • アプリケーション適用あり: ELISA, IHC-P, WB, IHC-Frmore details
  • 種交差性
    交差種: Sheep, Rabbit, Cow, Dog, Human, Pig, Kangaroo
    非交差種: Mouse, Rat, Chicken, Guinea pig
  • 免疫原

    Acid-digested pepsin soluble Dog Type V Collagen.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 97% Phosphate Buffer, 2.9% Sodium chloride
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名1E2-E4/Col5
  • ミエローマNS1/1-Ag4-1
  • アイソタイプIgG2a
  • 軽鎖の種類kappa

アプリケーション

Our Abpromise guarantee covers the use of ab112551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/50.
ab112551 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 184 kDa.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

Anti-Collagen Type V antibody [1E2-E4/Col5] (ab112551) 使用論文

ab112551 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Pig Tissue lysate - whole (Heart)
Specification Heart
Treatment 5 pigs are treated with pacemaker for 14 days (pm)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jan 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"