製品の概要

  • 製品名
    Anti-Collagen IV antibody [1043]
    Collagen IV 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1043] to Collagen IV
  • アプリケーション
    適用あり: WB, IHC-P, IHC-Fr, ICC, Flow Cytmore details
  • 種交差性
    交差種: Human
    非交差種: Rat, Rabbit, Cow, Pig
  • 免疫原

    Full length protein corresponding to Human Collagen IV.

  • 特記事項

    In IHC, recognizes the extracellular – basement membranes.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 27th October 2017. The following lots are still unpurified and still in stock as of 27th October 2017- GR275641-7, GR275641-13, and GR275641-14. Lot numbers other than GR275641-7, GR275641-13, and GR275641-14 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/1000. Use under non reducing condition. Predicted molecular weight: 160 kDa.
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC 1/50 - 1/100. Methanol fixed.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
    Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
  • 組織特異性
    Highly expressed in placenta.
  • 関連疾患
    Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
    Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
    Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
  • 配列類似性
    Belongs to the type IV collagen family.
    Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
  • ドメイン
    Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
  • 翻訳後修飾
    Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
    The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
    Proteolytic processing produces the C-terminal NC1 peptide, arresten.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Arresten antibody
    • BSVD antibody
    • CO4A1_HUMAN antibody
    • COL4A1 antibody
    • collagen alpha-1(IV) chain antibody
    • collagen type IV alpha 1 chain antibody
    • RATOR antibody
    see all

画像

  • ab86042 (TCS version) staining Collagen IV in Human kidney tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

  • Immunohistochemistry on frozen section of Human colon with ab86042 (TCS version).

  • Immunohistochemistry on paraffin section of Human skin

参考文献

This product has been referenced in:
  • Henshaw FR  et al. Topically applied connective tissue growth factor/CCN2 improves diabetic preclinical cutaneous wound healing: potential role for CTGF in human diabetic foot ulcer healing. J Diabetes Res 2015:236238 (2015). Read more (PubMed: 25789327) »
  • Havenith MG  et al. Human specific anti-type IV collagen monoclonal antibodies, characterization and immunohistochemical application. Histochemistry 87:123-8 (1987). Read more (PubMed: 3305430) »

See all 2 Publications for this product

レビューと Q&A

Your interpretation of the information is correct. It has been found with this antibody that it can be used to detect the denatured protein but not with a reducing agent present. This is illustrated in the following reference:

Havenith MG et a...

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Thank you for contacting us.

This antibody has not been tested in rat. A BLAST search of the immunogen showed 84% homology with the rat sequence, so it is likely to cross-react.

I hope this information is helpful to you. Please do...

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Thank you for clarifying that you would prefer to do IHC on paraffin tissue sections rather than frozen tissue sections. For both of these applications I would indeed suggest checking the biocompare website. Ideally you would need a mouse anti-rabbit c...

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I am sorry, I was under the impression that you wanted to do immunohistochemistry on rabbit tissue. If this is not the case, what application would you like to use the anti-collagen IV antibody in?

With regards to finding an antibody which is...

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As previously discussed with you, ab6311, has been shown to not cross react with a number of different species but this does not necessarily mean that it will not cross react with mouse. It is regrettably very difficult to suggest antibodies to custome...

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Thank you for contacting us in regards to anti-Collagen IV antibodies.

As discussed over the phone, we do not currently have any antibodies which have been shown to detect human Collagen IV but not the mouse protein in Western blotting (or an...

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\It is recommended that 0.1 mM CaCl2 and 0.1 mM MgCl2 should be included in the primary antibody incubation buffer. If the initial antibody incubation buffer did not contain 0.1 mM CaCl2 and 0.1 mM MgCl2, I think it is worthwhile to try the staining on...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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