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Full length native protein (purified) corresponding to Cow Collagen I.
Database link: P02452
IHC-P application: We have received positive as well as negative customer feedback for IHC-P. The antibody is not batch-tested in this application, thus additional troubleshooting might be needed to obtain satisfactory results.
Our Abpromise guarantee covers the use of ab90395 in the following tested applications.
|Dot blot||Use at an assay dependent concentration.
|IHC-Glut||Use at an assay dependent concentration.|
|IHC-R||Use at an assay dependent concentration.|
|Indirect ELISA||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Use under non reducing condition. Predicted molecular weight: 139 kDa.
The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions, as the antibody does not recognise denatured protein.
|ELISA||Use at an assay dependent concentration.
|Electron Microscopy||1/100 - 1/1000. PubMed: 17016762for Gold labeling|
|IHC-FoFr||1/100. PubMed: 17016762Fix in Zamboni's solution (2% paraformaldehyde, 0.2% picric acid in phosphate-buffered saline (PBS), pH 7.6) for 2 h at 4C, store in 20% sucrose in 0.5 mM PBS at 4C.|
ab90395 staining Collagen I in Pig Ureter tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/2000) for 4 hours at 2°C. A Cy2®-conjugated Goat anti-mouse polyclonal (1/800) was used as the secondary antibody.