製品の概要
- 製品名Anti-Collagen I antibody [COL-1]
Collagen I 一次抗体 製品一覧 - 製品の詳細Mouse monoclonal [COL-1] to Collagen I
- 特異性Recognises the native (helical) form of collagen type I using ELISA and dot-blot. Does not react with the thermally-denatured molecule. The antibody shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI. Strong staining of connective tissue fibres is seen in acetone-fixed or unfixed frozen sections.
- アプリケーション適用あり: IHC-Fr, Dot blot, ICC/IF, Electron Microscopy, Indirect ELISA, ELISA, WB, IHC-FoFrmore details
- 種交差性交差種: Mouse, Rat, Rabbit, Cow, Cat, Dog, Human, Pig, Monkey, Rhesus monkey, Deer
- 免疫原
Full length native protein (purified) corresponding to Cow Collagen I.
- エピトープThe epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding. There have been varying results when using this antibody in IHC-P. Please refer to our customer Abreviews for more protocol information and optimization steps when using this antibody in IHC-P.
- ポジティブ･コントロール
- WB: total pig skin lysate, human kidney lysate (see reviews)
- 特記事項
Production of this antibody has been changed on 23rd June 2016. The following lots are from ascites and are still in stock as of 23rd June 2016 : GR210978, GR175242, GR158374. Lot numbers higher than GR210978 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- バッファーpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... - 精製度Proprietary Purification
- 特記事項（精製）Purified from Tissue culture supernatant.
- ポリ/モノモノクローナル
- クローン名COL-1
- アイソタイプIgG1
- 研究分野
関連製品
- Compatible Secondaries
- Positive Controls
- Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab6308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use a concentration of 3.5 - 7 µg/ml. (amplification required).Use on unfixed tissue or acetone fixed tissue. |
|
| Dot blot | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 17230415 | |
| Electron Microscopy | Use at an assay dependent concentration. PubMed: 17016762 | |
| Indirect ELISA | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa). The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions. |
|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 17016762 Fix in Zamboni's solution (2% paraformaldehyde, 0.2% picric acid in phosphate-buffered saline (PBS), pH 7.6) for 2 h at 4C, store in 20% sucrose in 0.5 mM PBS at 4C. |
ターゲット情報
- 機能Type I collagen is a member of group I collagen (fibrillar forming collagen).
- 組織特異性Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
- 関連疾患Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. - 配列類似性Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. - 翻訳後修飾Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. - 細胞内局在Secreted > extracellular space > extracellular matrix.
- Information by UniProt
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参照データベース
- Entrez Gene: 282187 Cow
- Entrez Gene: 282188 Cow
- Entrez Gene: 403651 Dog
- Entrez Gene: 403824 Dog
- Entrez Gene: 1277 Human
- Entrez Gene: 1278 Human
- Entrez Gene: 12842 Mouse
- Entrez Gene: 12843 Mouse
see all -
別名
- Alpha 1 type I collagen antibody
- Alpha 2 type I collagen antibody
- alpha 2 type I procollagen antibody
see all
Anti-Collagen I antibody [COL-1] 画像
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![Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)](http://a.abcam.com/ps/products/6/ab6308/Images/ab6308_1.jpg)
Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)This image was kindly supplied as part of the review submitted by Arvi-Matti KuusniemiImmunoperoxidase staining of unfixed frozen tissue sections with ab6308. Picture of human kidney cortex showing two glomeruli and surrounding tubulointerstitium.
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![Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [COL-1] (ab6308)](http://a.abcam.com/ps/products/6/ab6308/Images/Collagen-I-Primary-antibodies-ab6308-6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [COL-1] (ab6308)This image was kindly supplied by Dr Sooneon Bae by Abreviewab6308 at a 1/1000 dilution staining in rabbit polymer scaffold with stem cells by Immunocytochemistry/ Immunofluorecence, incubated for 2 hours at 20°C. PFA fixed. Permeabilized using 0.5% Triton X-100. Blocked with 3% BSA for 2 hours at 4°C. Secondary used at 1/1000 dilution monoclonal donkey anti-mouse IgG (H+L) conjugated to FITC.1st column: DAPI stain (blue)2nd column: collagen type I (green)3rd column: merge image
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![Western blot - Anti-Collagen I antibody [COL-1] (ab6308)](http://a.abcam.com/ps/products/6/ab6308/Images/Collagen-I-Primary-antibodies-ab6308-4.jpg)
Western blot - Anti-Collagen I antibody [COL-1] (ab6308)This image is courtesy of an anonymous AbreviewAnti-Collagen I antibody [COL-1] (ab6308) at 1/1000 dilution + Human brain whole tissue lysate at 10 µg
Secondary
An HRP-conjugated goat polyclonal to mouse IgG at 1/7500 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 130 kDa
Exposure time : 24 hoursThis image is courtesy of an anonymous Abreview
Anti-Collagen I antibody [COL-1] (ab6308) 使用論文
This product has been referenced in:
- Baumann S & Hennet T Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1 Collagen Galactosyltransferase. J Biol Chem 291:18514-24 (2016). Read more (PubMed: 27402836) »
- Li L et al. Thymic Stromal Lymphopoietin Promotes Fibrosis and Activates Mitogen-Activated Protein Kinases in MRC-5 Cells. Med Sci Monit 22:2357-62 (2016). WB ; Human . Read more (PubMed: 27385084) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"