製品の概要

  • 製品名Anti-Collagen I antibody (Biotin)
    Collagen I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Collagen I (Biotin)
  • 標識Biotin
  • 特異性Negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • アプリケーション適用あり: IHC-P, ELISA, IHC-Fr, Immunomicroscopy, IP, WBmore details
  • 種交差性
    交差種: Cow, Human
    交差が予測される動物種: all Mammals
  • 免疫原

    Collagen Type I from adult human knee cartilage and bovine nasal cartilage.

  • 特記事項At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.


    This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 8
    Preservative: 0.01% Sodium azide
    Constituents: 0.44% Sodium chloride, 1% BSA, 0.15% EDTA, 4.8% Sodium borate
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • 一次抗体 備考This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/200.
ELISA Use at an assay dependent concentration. 1/3000 - 1/6000.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
IP Use at an assay dependent concentration. 1/100.
WB Use at an assay dependent concentration. 1/3000 - 1/6000.

ターゲット情報

  • 機能Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • 組織特異性Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • 関連疾患Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • 配列類似性Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • 翻訳後修飾Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Anti-Collagen I antibody (Biotin) 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue sections at pH9 labeling Collagen I with ab6577 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1/10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

  • Flow Cytometry analysis of primary adult human dermal fibroblast cells labeling Collagen I with ab6577 5µg/mL for 45 min at 4°C. Secondary antibody: Rabbit Streptavidin, R-PE antibody at 1/500 for 15 min at RT.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue sections at pH6 labeling Collagen I with ab6577 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1/10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

Anti-Collagen I antibody (Biotin) (ab6577) 使用論文

This product has been referenced in:
  • Jarman ER  et al. A translational preclinical model of interstitial pulmonary fibrosis and pulmonary hypertension: mechanistic pathways driving disease pathophysiology. Physiol Rep 2:N/A (2014). Flow Cyt ; Rat . Read more (PubMed: 25214520) »
  • Wen L  et al. Mild Electrical Pulse Current Stimulation Upregulates S100A4 and Promotes Cardiogenesis in MSC and Cardiac Myocytes Coculture Monolayer. Cell Biochem Biophys 65:43-55 (2013). Read more (PubMed: 22941361) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry. You are correct, this ab34710 probably will not react well with your aged and denatured samples. This antibody will only bind with the intact 3D protein, so no denaturants can be used in the gel or in the buffers. I hope...

Read More

Thank you for your email. What did your sample buffer consist of? To use ab6577 in Western blotting, you must use non-denaturing, non-dissociating Western blotting conditions. Please follow the suggestions below in order to use this antibody in Western...

Read More

Please reference the book: Gel Electrophoresis of Proteins, A Practical Approach, edited by B.D. Hames and D. Rickwood.

This antibody has been shown to react with collagen type 1 in human and bovine using immunohistochemistry on frozen tissue sections.

The epitope recognized by ab8308 is sensitive to routine formalin fixation and paraffin embedding. Strong staining of connective tissue fibres is seen in acetone-fixed or unfixed frozen sections. So yes, this antibody has been tested with PES but has b...

Read More

Yes, ab6577 is a biotinylated version of ab292

This antibody should work with ethanol or acetone in frozen tissue.

Anti-collagen should react with mouse and rat.

We use pepsin digestion and salt precipitation, not peptides as antigens for our anti-collagens. We do not have any information concerning the nature of the epitope that the antibody is recognising. We believe that these antibodies have not yet been...

Read More

Sorry, they have not been tested in immunohistochemistry on mouse tissue.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"