製品の概要

  • 製品名Anti-Collagen I antibody [5D8-G9]
    Collagen I 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5D8-G9] to Collagen I
  • 特異性Ab23446 is highly specific for type I collagen. The native triple-helical conformation is required for reaction. It has no cross reactivity with type II, III, V and VI collagens. There is no evidence for cross reactivity with other connnective tissue proteins (laminin, fibronectin, elastin).
  • アプリケーション適用あり: ELISA, ICC/IF, IP, Flow Cyt, WB, IHC-Frmore details
  • 種交差性
    交差種: Sheep, Goat, Cow, Dog, Human, Pig
    非交差種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Kangaroo
  • 免疫原

    Full length native protein (purified) corresponding to Human Collagen I.
    Database link: P02452

  • エピトープThe epitope recognised by ab23446 is located near the C terminal of the molecule.
  • ポジティブ・コントロール
    • human and bovine skin

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
ICC/IF Use at an assay dependent concentration. PubMed: 20161070
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 20161070ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 134 kDa.

Conditions need to be native to detect Collagen I with this monoclonal antibody, hence would only detect non-denatured, non-reduced collagen type 1.

IHC-Fr Use at an assay dependent concentration. Note: If fixation of tissue is required, acetone or ethanol is recommended.

ターゲット情報

  • 機能Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • 組織特異性Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • 関連疾患Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • 配列類似性Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • 翻訳後修飾Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Anti-Collagen I antibody [5D8-G9] 画像

  • Immunohistochemistry (Frozen sections) analysis of sheep vagina tissue labelling Collagen I with ab23446.

  • Immunohistochemistry (Frozen sections) analysis of human endometrium tissue labelling Collagen I with ab23446.

Anti-Collagen I antibody [5D8-G9] (ab23446) 使用論文

This product has been referenced in:
  • Jurgens WJ  et al. One-step surgical procedure for the treatment of osteochondral defects with adipose-derived stem cells in a caprine knee defect: a pilot study. Biores Open Access 2:315-25 (2013). IHC ; Goat . Read more (PubMed: 23914338) »
  • Flagler DJ  et al. Intracellular Flow Cytometric Measurement of Extracellular Matrix Components in Porcine Intervertebral Disc Cells. Cell Mol Bioeng 2:264-273 (2009). Flow Cyt, ICC/IF ; Pig . Read more (PubMed: 20161070) »

See all 5 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Goat Tissue sections (Bone and Hyaline Cartilage)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: protease
Permeabilization Yes - PBS Tween20
Specification Bone and Hyaline Cartilage
Fixative Alcohol, formalin and acid acetic
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Abcam user community

Verified customer

投稿 Jul 12 2016

Thank you for contacting Abcam

I found one anti-Collagen I antibody that has been tested in Flow cytometry and would be backed by our AbPromise guarantee to work against human sample in flow cytometry for 6 months after purchase. However, this...

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DISCOUNT CODE:

100%ABR-T71OF für ab23446
100%ABR-T71OS für ab82354

Ablaufdatum: 31. August 2012

Es freut uns zu hören, dass Sie unser Angebot unsere Antikörper ab23446 und ab82354 inRatteund Schwei...

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Vielen Dank für Ihren Anruf.

Wie versprochen habe ich unsere Kollagen-Antikörper nach den von Ihnen genannten Kriteriengeprüft. Leider habe ich keinen perfekten Treffer gefunden, dafür aber vielleicht ein paar Kandidaten, ...

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Thank you for your enquiry.

I'm happy to help answer your questions:

1. I can recommend the following collagen I antibody, tested in WB and pig samples, and also this should be suitable for denaturing conditions. I can recommend t...

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Gracias por contactarnos.

Para la detección del colágeno I en borrego mediante WB, el anticuerpo ab23446 está testado y garantizado por nuestra garantía Abpromise:

http://www.abcam.com/Collagen-I-antibody...

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Thank you for contacting us.

We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:

Click here (or use the following: http://www.abcam.com/index.html?datasheet=52460).

For collagen I, we have onl...

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Abreviews
Application ELISA
Sample Human Purified protein (Human Collagen Type I)
Specification Human Collagen Type I
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Type Sandwich (Detection)
Username

Abcam user community

Verified customer

投稿 Jun 12 2008

Thank you for your enquiry. I have received the following protocol details from the source of this antibody: They recommend the cutting of fixed (using methanol or acetone) 6 um thick frozen sections, incubating with the antibody, washing twice...

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Thank you for your enquiry. The native triple-helical conformation is required for reaction. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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