製品の概要

  • 製品名Anti-COL4A3BP antibody
    COL4A3BP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to COL4A3BP
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide from a region between residue 1 and 50 of human COL4A3BP (NP_005704.1)

  • ポジティブ・コントロール
    • Whole cell lysate from Hela and 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 1-4µg/mg of lysate.
    WB: 1/2500 - 1/10000. Detects bands of approximately 70, 71, 110 and 230 kDa (predicted molecular weight: 71 and 70 kDa for isoforms 1 and 2 respectively).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能May mediate the intracellular trafficking of ceramide in a non-vesicular manner.
    • 組織特異性Widely expressed.
    • 配列類似性Contains 1 PH domain.
      Contains 1 START domain.
    • ドメインThe START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
      The PH domain targets the Golgi apparatus.
      The FFAT motif is required for interaction with VAPA and VAPB.
    • 翻訳後修飾Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
    • 細胞内局在Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alpha 3 type IV collagen binding protein antibody
      • C43BP_HUMAN antibody
      • Ceramide transfer protein antibody
      • Ceramide transporter antibody
      • CERT antibody
      • Cert protein antibody
      • CERTL antibody
      • col4a3bp antibody
      • Collagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • Collagen type IV alpha 3 binding protein antibody
      • Collagen type IV alpha-3-binding protein antibody
      • FLJ20597 antibody
      • Goodpasture antigen binding protein antibody
      • Goodpasture antigen-binding protein antibody
      • GPBP antibody
      • hCERT antibody
      • Lipid transfer protein CERTL antibody
      • Procollagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • StAR related lipid transfer (START) domain containing 11 antibody
      • StAR related lipid transfer protein 11 antibody
      • StAR-related lipid transfer protein 11 antibody
      • StARD 11 antibody
      • StARD11 antibody
      • START domain containing 11 antibody
      • START domain containing protein 11 antibody
      • START domain-containing protein 11 antibody
      see all

    Anti-COL4A3BP antibody 画像

    • All lanes : Anti-COL4A3BP antibody (ab72534) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg


      Predicted band size : 71 kDa
      Observed band size : 70,71 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 110 kDa,230 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of COL4A3BP.
      Lane 1: ab72534 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72534 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 30 seconds.

    Anti-COL4A3BP antibody (ab72534) 使用論文

    ab72534 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab72534.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"