製品の概要

  • 製品名
    Anti-COL22A1 antibody
  • 製品の詳細
    Rabbit polyclonal to COL22A1
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    antigen sequence: LKEELEEIAS EPKSAHVFHV SDFNAIDKIR GKLRRRLCEN VLCPSVRVEG DRFKHTNGGT KEITGFDLMD LFSVKEILGK RENGAQSSYV RMG, corresponding to amino acids 181-273 of Human COL22A1.

  • ポジティブ・コントロール
    • Human urinary bladder tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Acts as a cell adhesion ligand for skin epithelial cells and fibroblasts.
  • 組織特異性
    Restrictive expression is observed at tissue junctions such as the myotendinous junction in skeletal and heart muscle, the articular cartilage-synovial fluid junction, or the border between the anagen hair follicle and the dermis in the skin. It is deposited in the basement membrane zone of the myotendinous junction and the hair follicle and associated with the extrafibrillar matrix in cartilage.
  • 配列類似性
    Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
    Contains 16 collagen-like domains.
    Contains 1 TSP N-terminal (TSPN) domain.
    Contains 1 VWFA domain.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2310067L16Rik antibody
    • C80743 antibody
    • COL22A1 antibody
    • Collagen alpha-1(XXII) chain antibody
    • COMA1_HUMAN antibody
    • Procollagen, type XXII, alpha 1 antibody
    see all

Anti-COL22A1 antibody 画像

  • ab121846 staining COL22A1 in Mouse muscle tendon junction tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% triton X-100 in PBS and blocked with 5% milk for 30 minutes; antigen retrieval was by heat mediation in tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An Alexa Fluor ® 594-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab121846 staining COL22A1 in mouse muscle tendon junction (knee muscle) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and permeabilized with 0.2% Triton X-100 in PBS; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An undiluted biotin-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab121846 at 1/250 dilution staining COL22A1 in Paraffin-embedded Human urinary bladder tissue by Immunohistochemistry. Note: strong cytoplasmic positivity in urothelial cells.

プロトコール

Anti-COL22A1 antibody (ab121846) 使用論文

ab121846 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample
Mouse Tissue sections (mouse muscle tendon junction (knee muscle))
Specification
mouse muscle tendon junction (knee muscle)
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Mrs. Maria Cecilia Ybanez

Verified customer

投稿 Jan 23 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample
Mouse Tissue sections (muscle tendon junction)
Specification
muscle tendon junction
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

投稿 Nov 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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