Anti-COL22A1 抗体 (ab121846)
Key features and details
- Rabbit polyclonal to COL22A1
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-COL22A1 antibody -
製品の詳細
Rabbit polyclonal to COL22A1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human COL22A1 aa 150-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8NFW1 -
ポジティブ・コントロール
- IHC-P: Human stomach and small intestine tissues; ICC: Human cell line U-2 OS.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121846の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P | (3) |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Acts as a cell adhesion ligand for skin epithelial cells and fibroblasts. -
組織特異性
Restrictive expression is observed at tissue junctions such as the myotendinous junction in skeletal and heart muscle, the articular cartilage-synovial fluid junction, or the border between the anagen hair follicle and the dermis in the skin. It is deposited in the basement membrane zone of the myotendinous junction and the hair follicle and associated with the extrafibrillar matrix in cartilage. -
配列類似性
Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
Contains 16 collagen-like domains.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 1 VWFA domain. -
細胞内局在
Secreted > extracellular space > extracellular matrix. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 169044 Human
- Omim: 610026 Human
- SwissProt: Q8NFW1 Human
- Unigene: 117169 Human
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別名
- 2310067L16Rik antibody
- C80743 antibody
- COL22A1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling COL22A1 with ab121846 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry analysis of human cell line U-2 OS cells labelling COL22A1 with ab121846 at 2 µg/mL
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling COL22A1 with ab121846 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling COL22A1 with ab121846 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling COL22A1 with ab121846 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab121846 は 4 報の論文で使用されています。
- Zhang Y et al. Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. Nat Commun 13:2447 (2022). PubMed: 35508470
- Pan R et al. A novel immune cell signature for predicting osteosarcoma prognosis and guiding therapy. Front Immunol 13:1017120 (2022). PubMed: 36189307
- Ishibashi K et al. Single-cell transcriptome analysis reveals cellular heterogeneity in mouse intra- and extra articular ligaments. Commun Biol 5:1233 (2022). PubMed: 36371589
- Scott RW et al. Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct Functions and Fates in Skeletal Muscle Regeneration. Cell Stem Cell 25:797-813.e9 (2019). PubMed: 31809738