Anti-Cofilin 抗体 [AF2C2] (ab75510)

製品の概要

  • 製品名
    Anti-Cofilin antibody [AF2C2]
    Cofilin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AF2C2] to Cofilin
  • アプリケーション
    適用あり: ChIP, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant full length Cofilin protein (Human) purified from E.coli (His-Cofilin)

  • ポジティブ・コントロール
    • Jurkat, Raji, SK-N-MC, PC12 and NIH3T3 cell lysates
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR166582 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
WB Use a concentration of 4 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • 組織特異性
    Widely distributed in various tissues.
  • 配列類似性
    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • 翻訳後修飾
    Phosphorylated on Ser-3 in resting cells.
  • 細胞内局在
    Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

画像

  • All lanes : Anti-Cofilin antibody [AF2C2] (ab75510) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : SK-N-MC cell lysate
    Lane 4 : PC12 cell lysate
    Lane 5 : NIH3T3 cell lysate


    Predicted band size : 18 kDa
    Observed band size : 18 kDa

プロトコール

参考文献

ab75510 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us with your question.

The exact epitope that this antibody recognizes has not yet been identified. Since ab75510 is a monoclonal antibody raised against the full length human cofilin protein, the epitope could lie an...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab75510. To check the status of the order please contact our Customer...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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