Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Widely distributed in various tissues.
Belongs to the actin-binding proteins ADF family. Contains 1 ADF-H domain.
Phosphorylated on Ser-3 in resting cells.
Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
Western blot of jurkat cells expressing cofilin 1. Blots were treated with (lanes 2, 3, 5 & 6) or without (lanes 1 & 4) lambda phosphatase for 1 hr at 37 degrees C. In lanes 3 & 6, phosphatase treatments were performed in the presence of phospho-cofilin 1 (Ser-3) peptide. Blots were probed with anti-phospho-Cofilin 1 (Ser-3) (lanes 1-3;) or anti-Cofilin 1 (lanes 4-6; ab29038). Phosphatase treatment removes Ser-3 phosphorylation of Cofilin, but this phosphatase activity is reduced in the presence of peptide.