製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cofilin
  • アプリケーション
    適用あり: IP, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Dog, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.

  • 特記事項
    The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB 1/10000. Predicted molecular weight: 19 kDa.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • 組織特異性
    Widely distributed in various tissues.
  • 配列類似性
    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • 翻訳後修飾
    Phosphorylated on Ser-3 in resting cells.
  • 細胞内局在
    Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

Anti-Cofilin antibody 画像

  • ICC/IF image of ab11062 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11062, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

Anti-Cofilin antibody (ab11062) 使用論文

This product has been referenced in:
  • Reinhard JR  et al. The calcium sensor Copine-6 regulates spine structural plasticity and learning and memory. Nat Commun 7:11613 (2016). Read more (PubMed: 27194588) »
  • Shao X  et al. Mechanical stimulation induces formin-dependent assembly of a perinuclear actin rim. Proc Natl Acad Sci U S A 112:E2595-601 (2015). Read more (PubMed: 25941386) »

See all 17 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (Brain)
Total protein in input
1000 µg
Specification
Brain
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

投稿 Sep 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (pancreatic cancer cell line)
Loading amount
20 µg
Specification
pancreatic cancer cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Hannah Whiteman

Verified customer

投稿 Jul 04 2008

Thank you for your phone call and I apologize for the shortage of ab11062 that you received. We are sending you a free of charge replacement vial of this antibody; it is on order reference# 111878. As the item is currently out of stock the estimated de...

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Thank you for your enquiry. When ab11062 was tested in IF with mouse NIH/3T3 fibroblasts, antigen retrieval was not performed. The cells were fixed with 3-4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Ab11062 was used at a dilution of 1...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HUVEC)
Specification
HUVEC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 %
Username

Abcam user community

Verified customer

投稿 Aug 15 2005

Thank you for your enquiry and your interest in our products. We regret to inform you that the cross-reactivity of ab11062 (cofilin 1) with cofilin 2 has not been tested yet. On the basis of poor sequence homology between human Cofilin 1 and Co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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