製品の概要

  • 製品名
    Anti-Cofilin 2 (phospho S3) antibody
    Cofilin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cofilin 2 (phospho S3)
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Chicken, Human
  • 免疫原

    Synthetic peptide: MASpGVTVNDE-C conjugated to KLH, corresponding to amino acids 1-10 of Human phospho-Cofilin 2 with C-terminal cysteine.

  • ポジティブ・コントロール
    • RIPA lysates from L6 cells, treated with LIM Kinase for 30 minutes.
  • 特記事項
    Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 30% Glycerol, 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 21 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • 組織特異性
    Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
  • 関連疾患
    Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.
  • 配列類似性
    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • 翻訳後修飾
    The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
  • 細胞内局在
    Nucleus matrix. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CFL 2 antibody
    • CFL2 antibody
    • COF2_HUMAN antibody
    • Cofilin 2 muscle antibody
    • Cofilin antibody
    • Cofilin muscle antibody
    • Cofilin muscle isoform antibody
    • Cofilin-2 antibody
    • Cofilin2 antibody
    • muscle isoform antibody
    • NEM 7 antibody
    • NEM7 antibody
    see all

プロトコール

Anti-Cofilin 2 (phospho S3) antibody (ab14134) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

rr/>Yes, I can confirm that this antibody is raised to recongize the cofilin 2 form which is phosphorylated at the serine in position 3.
I do not know to which extent the antibody does also recognize the unphosphorylated form. Please let me know i...

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To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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