製品の概要

  • 製品名Coenzyme A Detection Kit
    Coenzyme A キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • アッセイタイプQuantitative
  • 検出感度
    > 2.5 µM
  • 検出範囲
    2.5 µM - 250 µM
  • 全工程の試験時間
    1h 00m
  • 製品の概要

    Abcam's Coenzyme A Detection Kit provides an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (? = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of Coenzyme A is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-Coenzyme A. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.
  • 別名
    • CoA
    • CoASH
    • HSCoA

アプリケーション

Our Abpromise guarantee covers the use of ab102504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Coenzyme A Detection Kit 画像

  • Example of Standard Curve obtained using ab102504.

  • Example of Standard Curve obtained using ab102504.

プロトコール

Coenzyme A Detection Kit (ab102504) 使用論文

ab102504 has not yet been referenced specifically in any publications.

Product Wall

Thank you for the information, and the clarification. I am not sure we have any data for precision, which would tell us how well the assay could distinguish among differences. The low standard, 0.2 nmol per well in the fluorescent version of the assay,...

Read More

Thank you for your interest in the Coenzyme A assay, ab102504. The detection limit of the fluorescent assay is 2.5uM, so it will not be suitable for detection of your 1uM samples. I am waiting for the lab to confirm compatibility with the reducing agen...

Read More

Thank you for your inquiry. Unfortunately we have not specifically tested the compatibilities of these compounds with this kit, so we cannot say with certainty whether these will be compatible. The kit should provide up to 100 tests (in...

Read More

Thank you for your follow up question. The lab producing this kit informed us that unfortunately the reaction mechanism information is proprietary and hence we cannot list all details except the one on the data sheet. Regarding the second...

Read More

Thank you for your inquiry. Unfortunately we do not have any references for this product as yet. I would like however to reassure you that we do guarantee this product to work as stated on the datasheet. We do indeed guarantee all our products to w...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"