Coenzyme A Assay Kit (ab102504)
Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 2.5 µM
製品の概要
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製品名
Coenzyme A Assay Kit
Coenzyme A キット 製品一覧 -
サンプルの種類
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
アッセイタイプ
Quantitative -
検出感度
> 2.5 µM -
検出範囲
2.5 µM - 250 µM -
全工程の試験時間
1h 00m -
製品の概要
Abcam's Coenzyme A Assay Kit provides an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.
Visit our FAQs page for tips and troubleshooting.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K367 Coenzyme A (CoA) Colorimetric/Fluorometric Assay Kit. K367-100 is the same size as the 100 test size of ab102504.
Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests ACS Substrate 1 x 1ml Acyl CoA Enzyme Mix 1 vial Assay Buffer V 1 x 25ml CoA Converter Mix 1 unit CoA Standard 1 unit OxiRed Probe 1 unit -
研究分野
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関連性
Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of Coenzyme A is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-Coenzyme A. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. -
別名
- CoA
- CoASH
- HSCoA
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab102504 は 6 報の論文で使用されています。
- Chang X et al. Pilot trial on the efficacy and safety of pantethine in children with pantothenate kinase-associated neurodegeneration: a single-arm, open-label study. Orphanet J Rare Dis 15:248 (2020). PubMed: 32928263
- Le CH et al. Tafazzin deficiency impairs CoA-dependent oxidative metabolism in cardiac mitochondria. J Biol Chem 295:12485-12497 (2020). PubMed: 32665401
- Smith ER et al. TGF-ß1 modifies histone acetylation and acetyl-coenzyme A metabolism in renal myofibroblasts. Am J Physiol Renal Physiol N/A:N/A (2019). PubMed: 30623724
- Dieterich IA et al. Acetyl-CoA flux regulates the proteome and acetyl-proteome to maintain intracellular metabolic crosstalk. Nat Commun 10:3929 (2019). PubMed: 31477734
- Iuso A et al. Mutations in PPCS, Encoding Phosphopantothenoylcysteine Synthetase, Cause Autosomal-Recessive Dilated Cardiomyopathy. Am J Hum Genet 102:1018-1030 (2018). PubMed: 29754768
- Machado MV et al. Vitamin B5 and N-Acetylcysteine in Nonalcoholic Steatohepatitis: A Preclinical Study in a Dietary Mouse Model. Dig Dis Sci 61:137-48 (2016). PubMed: 26403427