製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CoCoA
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 650 and the C terminus (residue 691) of human CoCoA (NP_065949.1)

  • ポジティブ・コントロール
    • Whole cell lysate from: HeLa, 293T, NIH3T3, Ramos and MCF7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性
    Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1 mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions.
  • 細胞内局在
    Cytoplasm. Nucleus. Note: shuttles between nucleus and cytoplasm.
  • 参照データベース
  • 別名
    • Calcium binding and coiled coil domain 1 antibody
    • CALCOCO1 antibody
    • Calphoglin antibody
    • Coiled coil coactivator protein antibody
    • Coiled coil leucine zipper coactivator 1 antibody
    • Coiled coil transcriptional coactivator antibody
    • Inorganic pyrophosphatase activator antibody
    • KIAA1536 antibody
    • PP13275 antibody
    • Sarcoma antigen NY-SAR-3 antibody
    see all

画像

  • All lanes : Anti-CoCoA antibody (ab70564) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : NIH3T3 whole cell lysate
    Lane 4 : Ramos whole cell lysate
    Lane 5 : MCF7 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 77 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of human CoCoA by Immunoprecipitation in whole cell lysate from MCF7 cells (1 mg, 20% of immunoprecipitate loaded/lane), using ab70564 at 3 µg/mg of lysate (lane 2).
    CoCoA was also immunoprecipitated using another CoCoA antibody (ab70563) which recognizes an upstream epitope (lane 1).
    Lane 3: Control IgG immunoprecipitate.
    Subsequent blotting of immunoprecipitate for the detection of CoCoA was performed using 1µg/ml of ab70564 and 50µg of lysate.
    Detection was by chemiluminescence with an exposure time of 30 seconds.

参考文献

This product has been referenced in:

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