ab64465 at 4µg/ml staining COBLL1 in human kidney tissue, epithelial cells of renal tubule (indicated by arrows)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COBLL1 antibody (ab64465)Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview
ab64465 staining COBLL1 in human bladder cancer tissue by Immunohistochemistry (formaldehyde fixed sections). Tissue was fixed with formaldehyde, a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 1% BSA for 30 minutes at room temperature followed by incubation with the primary antibody, at a 1/80 dilution, for 1 hour. A HRP-conjugated goat anti-rabbit polyclonal was used as secondary antibody un-diluted. Note that the protein localizes in the cytoplasm.