製品の概要

  • 製品名
    Anti-Coatomer subunit delta antibody
    Coatomer subunit delta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Coatomer subunit delta
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 89 and 492 of Coatomer subunit delta (NP_001646).

  • ポジティブ・コントロール
    • WB: 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysates ICC/IF: HeLa cells

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.
    Contains 1 MHD (mu homology) domain.
  • 細胞内局在
    Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Archain 1 antibody
    • Archain antibody
    • Archain vesicle transport protein 1 antibody
    • Arcn1 antibody
    • Coatomer delta subunit antibody
    • Coatomer protein complex, subunit delta antibody
    • Coatomer protein delta COP antibody
    • Coatomer subunit delta antibody
    • COPD_HUMAN antibody
    • Delta coat protein antibody
    • Delta COP antibody
    • Delta-coat protein antibody
    • Delta-COP antibody
    see all

画像

  • All lanes : Anti-Coatomer subunit delta antibody (ab96725) at 1/500 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 58 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96725 at 1/200 dilution. Lower image merged with DNA probe.

参考文献

This product has been referenced in:
  • Sun E  et al. Dissecting the role of COPI complexes in influenza virus infection. J Virol 87:2673-85 (2013). Read more (PubMed: 23255804) »
  • Zhang X  et al. Dopamine receptor D3 regulates endocytic sorting by a Prazosin-sensitive interaction with the coatomer COPI. Proc Natl Acad Sci U S A 109:12485-90 (2012). WB ; Human . Read more (PubMed: 22802617) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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