Anti-Coatomer subunit delta/COPD 抗体 (ab96725)
Key features and details
- Rabbit polyclonal to Coatomer subunit delta/COPD
- Suitable for: WB, ICC/IF, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Coatomer subunit delta/COPD antibody -
製品の詳細
Rabbit polyclonal to Coatomer subunit delta/COPD -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Recombinant fragment corresponding to Human Coatomer subunit delta/COPD aa 89-492.
Database link: NP_001646 -
ポジティブ・コントロール
- WB: 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysates. ICC/IF: HeLa cells. IHC-P: Mouse brain tissue. IHC-Fr: Mouse cerebellum tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96725の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
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ICC/IF |
1/100 - 1/500.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IHC-Fr |
1/100 - 1/1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min. |
特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
ICC/IF
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IHC-Fr
1/100 - 1/1000. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min. |
ターゲット情報
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機能
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.
Contains 1 MHD (mu homology) domain. -
細胞内局在
Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. - Information by UniProt
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参照データベース
- Entrez Gene: 372 Human
- Entrez Gene: 213827 Mouse
- Entrez Gene: 300674 Rat
- Omim: 600820 Human
- SwissProt: P48444 Human
- SwissProt: Q5XJY5 Mouse
- SwissProt: Q66H80 Rat
- Unigene: 33642 Human
see all -
別名
- Archain 1 antibody
- Archain antibody
- Archain vesicle transport protein 1 antibody
see all
画像
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Frozen section of mouse cerebellum tissue stained for Coatomer subunit delta/COPD using ab96725 at 1/250 dilution (green). The nuclear counter stain is DAPI (blue). NF-H is detected with an anti NF-H antibody (red).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
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Paraffin-embedded mouse brain tissue stained for Coatomer subunit delta/COPD using ab96725 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
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Paraformaldehyde-fixed HeLa cells stained for Coatomer subunit delta/COPD using ab96725 at 1/500 dilution (green) in ICC/IF. Alpha tubulin filaments are detected with an anti-tubulin antibody (red).
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All lanes : Anti-Coatomer subunit delta/COPD antibody (ab96725) at 1/500 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96725 at 1/200 dilution. Lower image merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab96725 は 4 報の論文で使用されています。
- McMillan EA et al. A Genome-wide Functional Signature Ontology Map and Applications to Natural Product Mechanism of Action Discovery. Cell Chem Biol 26:1380-1392.e6 (2019). PubMed: 31378711
- Izumi K et al. ARCN1 Mutations Cause a Recognizable Craniofacial Syndrome Due to COPI-Mediated Transport Defects. Am J Hum Genet 99:451-9 (2016). PubMed: 27476655
- Sun E et al. Dissecting the role of COPI complexes in influenza virus infection. J Virol 87:2673-85 (2013). PubMed: 23255804
- Zhang X et al. Dopamine receptor D3 regulates endocytic sorting by a Prazosin-sensitive interaction with the coatomer COPI. Proc Natl Acad Sci U S A 109:12485-90 (2012). WB ; Human . PubMed: 22802617