製品の概要

  • 製品名
    Anti-COASY (phospho S182) antibody
    COASY 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to COASY (phospho S182)
  • 特異性
    This antibody is specific for phospho S182 COASY
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human COASY, phosphorylated at S182.

    (Peptide available as ab46197.)

  • ポジティブ・コントロール
    • HEK293 cells transiently transfected with wt CoA synthase (COASY)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: Use at an assay dependent dilution. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa) in HEK293 cells transiently transfected with CoA synthase

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
    • 組織特異性
      Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.
    • パスウェイ
      Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.
      Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.
    • 配列類似性
      In the central section; belongs to the eukaryotic coaD family.
      Contains 1 DPCK (dephospho-CoA kinase) domain.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Bifunctional coenzyme A synthase antibody
      • Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase antibody
      • CoA synthase antibody
      • COASY antibody
      • COASY_HUMAN antibody
      • Coenzyme A synthase antibody
      • Dephospho-CoA kinase antibody
      • Dephospho-CoA pyrophosphorylase antibody
      • Dephosphocoenzyme A kinase antibody
      • DPCK antibody
      • DPCOAK antibody
      • NBP antibody
      • Nucleotide binding protein antibody
      • Pantetheine-phosphate adenylyltransferase antibody
      • Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase antibody
      • pOV 2 antibody
      • POV-2 antibody
      • POV2 antibody
      • PPAT antibody
      • UKR1 antibody
      see all

    画像



    • Predicted band size : 62 kDa


      Western blot analysis of total cell extracts from HEK293 cells transiently transfected with wt and S182A mutant of CoA synthase (CoAsy). This shows that ab45994 specifically recognize CoA synthase phosphorylated at S182. The bottom panel shows the positive loading control b-actin

    プロトコール

    参考文献

    ab45994 has not yet been referenced specifically in any publications.

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