製品の概要

  • 製品名Anti-CNTROB antibody
    CNTROB 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to CNTROB
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    CNTROB (AAH21134.1, 1 a.a. ~ 903 a.a) full-length human protein.

  • ポジティブ・コントロール
    • CNTROB transfected 293T lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 20861312
WB 1/500 - 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 102 kDa).

ターゲット情報

  • 機能Required for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis.
  • 組織特異性Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
  • 発生段階Preferentially incorporated into the newly assembled daughter centriole during centriole assembly at the late G1 or early S phase. Remains in the daughter centrioles throughout the cell cycle. At the next cycle of centriole duplication, its amount on the original daughter centriole eventually decreases.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome > centriole. Centriole-associated, asymmetrically localizes to the daughter centriole.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 9830165K03Rik antibody
    • Centrobin antibody
    • centrobin, centrosomal BRCA2 interacting protein antibody
    • Centrosomal BRCA2 interacting protein antibody
    • Centrosomal BRCA2-interacting protein antibody
    • CNTRB_HUMAN antibody
    • Cntrob antibody
    • LIP8 antibody
    • LYST interacting protein 8 antibody
    • LYST-interacting protein 8 antibody
    • LYST-interacting protein LIP8 antibody
    • PP1221 antibody
    • RGD1307488 antibody
    • RP23 26L6.9 antibody
    see all

Anti-CNTROB antibody 画像

  • All lanes : Anti-CNTROB antibody (ab70448) at 1/500 dilution

    Lane 1 : CNTROB transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 102 kDa
    Observed band size : 135 kDa (why is the actual band size different from the predicted?)

Anti-CNTROB antibody (ab70448) 使用論文

This product has been referenced in:
  • Shiratsuchi G  et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34:97-114 (2015). Human . Read more (PubMed: 25385835) »
  • Kong D  et al. Centriole maturation requires regulated Plk1 activity during two consecutive cell cycles. J Cell Biol 206:855-65 (2014). Read more (PubMed: 25246616) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"